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Forward genetic screens identify a role for the mitochondrial HER2 in E-2-hexenal responsiveness

Overview of attention for article published in Plant Molecular Biology, September 2017
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Title
Forward genetic screens identify a role for the mitochondrial HER2 in E-2-hexenal responsiveness
Published in
Plant Molecular Biology, September 2017
DOI 10.1007/s11103-017-0659-8
Pubmed ID
Authors

Alessandra Scala, Rossana Mirabella, Joachim Goedhart, Michel de Vries, Michel A. Haring, Robert C. Schuurink

Abstract

This work adds a new player, HER2, downstream of the perception of E-2-hexenal, a green leaf volatile, and shows that E-2-hexenal specifically changes the redox status of the mitochondria. It is widely accepted that plants produce and respond to green leaf volatiles (GLVs), but the molecular components involved in transducing their perception are largely unknown. The GLV E-2-hexenal inhibits root elongation in seedlings and, using this phenotype, we isolated E-2-hexenal response (her) Arabidopsis thaliana mutants. Using map-based cloning we positioned the her2 mutation to the At5g63620 locus, resulting in a phenylalanine instead of serine on position 223. Knockdown and overexpression lines of HER2 confirmed the role of HER2, which encodes an oxidoreductase, in the responsiveness to E-2-hexenal. Since E-2-hexenal is a reactive electrophile species, which are known to influence the redox status of cells, we utilized redox sensitive GFP2 (roGFP2) to determine the redox status of E-2-hexenal-treated root cells. Since the signal peptide of HER2 directed mCherry to the mitochondria, we targeted the expression of roGFP2 to this organelle besides the cytosol. E-2-hexenal specifically induced a change in the redox status in the mitochondria. We did not see a difference in the redox status in her2 compared to wild-type Arabidopsis. Still, the mitochondrial redox status did not change with Z-3-hexenol, another abundant GLV. These results indicate that HER2 is involved in transducing the perception of E-2-hexenal, which changes the redox status of the mitochondria.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 13 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 4 31%
Professor > Associate Professor 2 15%
Unspecified 2 15%
Researcher 2 15%
Student > Doctoral Student 1 8%
Other 2 15%
Readers by discipline Count As %
Agricultural and Biological Sciences 7 54%
Biochemistry, Genetics and Molecular Biology 3 23%
Unspecified 3 23%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 September 2017.
All research outputs
#10,443,662
of 11,779,542 outputs
Outputs from Plant Molecular Biology
#2,169
of 2,328 outputs
Outputs of similar age
#224,963
of 265,979 outputs
Outputs of similar age from Plant Molecular Biology
#23
of 33 outputs
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