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A two-stage temperature control strategy enhances extracellular secretion of recombinant α-cyclodextrin glucosyltransferase in Escherichia coli

Overview of attention for article published in AMB Express, August 2017
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1 tweeter

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14 Mendeley
Title
A two-stage temperature control strategy enhances extracellular secretion of recombinant α-cyclodextrin glucosyltransferase in Escherichia coli
Published in
AMB Express, August 2017
DOI 10.1186/s13568-017-0465-3
Pubmed ID
Authors

Yang Li, Jia Liu, Yinglan Wang, Bingjie Liu, Xiaofang Xie, Rui Jia, Caiming Li, Zhaofeng Li

Abstract

The effects of temperature on extracellular secretion of the α-cyclodextrin glucosyltransferase (α-CGTase) from Paenibacillus macerans JFB05-01 by Escherichia coli were investigated. When protein expression was induced at constant temperature, the greatest amount of extracellular recombinant α-CGTase was produced at 25 °C. Higher or lower induction temperatures were not conducive to extracellular secretion of recombinant α-CGTase. To enhance extracellular secretion of α-CGTase by E. coli, a two-stage temperature control strategy was adopted. When expression was induced at 25 °C for 32 h, and then the temperature was shifted to 30 °C, the extracellular α-CGTase activity at 90 h was 45% higher than that observed when induction was performed at a constant temperature of 25 °C. Further experiments suggested that raising the induction temperature can benefit the transport of recombinant enzyme and compensate for the decreased rate of recombinant enzyme synthesis during the later stage of expression. This report provides a new method of optimizing the secretory expression of recombinant enzymes by E. coli.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 14 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 14 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 4 29%
Student > Doctoral Student 3 21%
Professor > Associate Professor 2 14%
Other 1 7%
Researcher 1 7%
Other 1 7%
Unknown 2 14%
Readers by discipline Count As %
Agricultural and Biological Sciences 5 36%
Engineering 2 14%
Biochemistry, Genetics and Molecular Biology 1 7%
Chemical Engineering 1 7%
Medicine and Dentistry 1 7%
Other 1 7%
Unknown 3 21%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 August 2017.
All research outputs
#7,252,685
of 11,648,047 outputs
Outputs from AMB Express
#301
of 692 outputs
Outputs of similar age
#150,601
of 263,265 outputs
Outputs of similar age from AMB Express
#19
of 41 outputs
Altmetric has tracked 11,648,047 research outputs across all sources so far. This one is in the 23rd percentile – i.e., 23% of other outputs scored the same or lower than it.
So far Altmetric has tracked 692 research outputs from this source. They receive a mean Attention Score of 2.2. This one is in the 6th percentile – i.e., 6% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 263,265 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 32nd percentile – i.e., 32% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 41 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.