| Title |
Assessment of packed bed bioreactor systems in the production of viral vaccines
|
|---|---|
| Published in |
AMB Express, April 2014
|
| DOI | 10.1186/s13568-014-0025-z |
| Pubmed ID | |
| Authors |
Ramya Rajendran, Rajendra Lingala, Siva Kumar Vuppu, Bala Obulapathi Bandi, Elaiyaraja Manickam, Sankar Rao Macherla, Stéphanie Dubois, Nicolas Havelange, Kapil Maithal |
| Abstract |
Vaccination is believed to be the most effective method for the prevention of infectious diseases. Thus it is imperative to develop cost effective and scalable process for the production of vaccines so as to make them affordable for mass use. In this study, performance of a novel disposable iCELLis fixed bed bioreactor system was investigated for the production of some viral vaccines like Rabies, Hepatitis-A and Chikungunya vaccines in comparison to conventional systems like the commercially available packed bed system and roller bottle system. Vero and MRC-5 cell substrates were evaluated for growth parameters in all the three systems maintaining similar seeding density, multiplicity of infection (MOI) and media components. It was observed that Vero cells showed similar growth in all the three bioreactors whereas MRC-5 cells showed better growth in iCELLis Nano system and roller bottle system. Subsequently, the virus infection and antigen production studies also revealed that for Hepatitis-A and Chikungunya iCELLis Nano bioreactor system was better to the commercial packed bed bioreactor and roller bottle systems. Although for rabies antigen production commercially available packed bed bioreactor system was found to be better. This study shows that different bioreactor platforms may be employed for viral vaccine production and iCELLis Nano is one of such new convenient and a stable platform for production of human viral vaccines. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 79 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 1% |
| Unknown | 78 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 18 | 23% |
| Student > Master | 9 | 11% |
| Student > Bachelor | 8 | 10% |
| Student > Ph. D. Student | 7 | 9% |
| Student > Doctoral Student | 4 | 5% |
| Other | 13 | 16% |
| Unknown | 20 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 10 | 13% |
| Agricultural and Biological Sciences | 10 | 13% |
| Engineering | 8 | 10% |
| Medicine and Dentistry | 5 | 6% |
| Chemical Engineering | 4 | 5% |
| Other | 19 | 24% |
| Unknown | 23 | 29% |
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 August 2018.
All research outputs
#7,199,583
of 22,754,104 outputs
Outputs from AMB Express
#156
of 1,231 outputs
Outputs of similar age
#70,611
of 226,860 outputs
Outputs of similar age from AMB Express
#4
of 27 outputs
Altmetric has tracked 22,754,104 research outputs across all sources so far. This one has received more attention than most of these and is in the 67th percentile.
So far Altmetric has tracked 1,231 research outputs from this source. They receive a mean Attention Score of 2.8. This one has done well, scoring higher than 85% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 226,860 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 67% of its contemporaries.
We're also able to compare this research output to 27 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 81% of its contemporaries.