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Adding value to rare tissue samples donated to biobanks: characterisation of breast tissue and primary cell cultures obtained from a female-to-male transgender patient

Overview of attention for article published in Cell and Tissue Banking, April 2014
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  • High Attention Score compared to outputs of the same age and source (88th percentile)

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1 tweeter

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Title
Adding value to rare tissue samples donated to biobanks: characterisation of breast tissue and primary cell cultures obtained from a female-to-male transgender patient
Published in
Cell and Tissue Banking, April 2014
DOI 10.1007/s10561-014-9444-y
Pubmed ID
Authors

Rebecca Millican-Slater, Rona Good, Claire Nash, Judith A. Heads, Steven Pollock, Rebecca Chalkley, Jenny Gomm, J. Louise Jones, Sreekumar Sundara-Rajan, Kieran Horgan, Andrew M. Hanby, Valerie Speirs

Abstract

Biobanks provide a window of opportunity to store and add value to material from rare cases allowing their future use in biomedical research. One such example is the opportunityto obtain good quality tissue from patients undergoing gender re-assignment. Following patient agreement to donate tissue samples to our biobank we catalogued the histological appearance, defined the expression of the hormone receptors ERα, PR, AR and the proliferation marker Ki67, and generated and characterised primary cell cultures in a female to male (FTM) transgender patient referred to our unit for surgery. Immunohistochemistry was performed for ERα, PR and AR and the proliferation marker Ki67. Hormone receptor expression was confined to epithelial cells lining the breast ducts. Ki67 immunoreactivity was sparse indicating little proliferation of luminal epithelium, consistent with normal mammary gland. Cultures of epithelial cells and fibroblasts were derived from surplus tissue. The latter lacked expression of epithelial markers and hormone receptors but exhibited expression of vimentin. Culture of the former on Matrigel saw an outgrowth of more rounded "epithelial-like" cells. Immunofluoresence characterisation showed a mixed phenotype with expression of vimentin and both myoepithelial and luminal epithelial markers. Sporadic weak ERα expression and moderate PR expression was seen. In summary, as well as routinely collecting tissue and blood samples, we have characterised and stored tissue and cells from a FTM transgender patient, adding value to this resource which,available from the Breast Cancer Campaign Tissue Bank for those interested in further studying the biology of FTM transgender tissue.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 14 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Netherlands 1 7%
Unknown 13 93%

Demographic breakdown

Readers by professional status Count As %
Researcher 4 29%
Student > Bachelor 4 29%
Professor 2 14%
Student > Doctoral Student 2 14%
Student > Master 1 7%
Other 1 7%
Readers by discipline Count As %
Medicine and Dentistry 7 50%
Psychology 1 7%
Neuroscience 1 7%
Pharmacology, Toxicology and Pharmaceutical Science 1 7%
Unknown 4 29%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 April 2014.
All research outputs
#10,918,919
of 13,734,991 outputs
Outputs from Cell and Tissue Banking
#108
of 159 outputs
Outputs of similar age
#127,652
of 189,241 outputs
Outputs of similar age from Cell and Tissue Banking
#1
of 9 outputs
Altmetric has tracked 13,734,991 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 159 research outputs from this source. They receive a mean Attention Score of 2.6. This one is in the 22nd percentile – i.e., 22% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 189,241 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 16th percentile – i.e., 16% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 9 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them