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Detection of bacterial sulfatase activity through liquid- and solid-phase colony-based assays

Overview of attention for article published in AMB Express, July 2017
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  • Good Attention Score compared to outputs of the same age and source (71st percentile)

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4 X users

Citations

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20 Mendeley
Title
Detection of bacterial sulfatase activity through liquid- and solid-phase colony-based assays
Published in
AMB Express, July 2017
DOI 10.1186/s13568-017-0449-3
Pubmed ID
Authors

Hey Young Yoon, Hyung Jun Kim, Soojin Jang, Jong-In Hong

Abstract

Bacterial arylsulfatases are crucial to biosynthesis in many microorganisms, as bacteria often utilize aryl sulfates as a source of sulfur. The bacterial sulfatases are associated with pathogenesis and are applied in many areas such as industry and agriculture. We developed an activity-based probe 1 for detection of bacterial sulfatase activity through liquid- and solid-phase colony-based assays. Probe 1 is hydrolyzed by sulfatase to generate fluorescent N-methyl isoindole, which is polymerized to form colored precipitates. These fluorescent and colorimetric properties of probe 1 induced upon treatment of sulfatases were successfully utilized for liquid-phase sulfatase activity assays for colonies and lysates of Klebsiella aerogenes, Mycobacterium avium and Mycobacterium smegmatis. In addition, probe 1 allowed solid-phase colony-based assays of K. aerogenes through the formation of insoluble colored precipitates, thus enabling accurate staining of target colonies under heterogeneous conditions.

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The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 20 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 20 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 5 25%
Student > Master 5 25%
Student > Ph. D. Student 2 10%
Student > Doctoral Student 2 10%
Student > Bachelor 1 5%
Other 3 15%
Unknown 2 10%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 5 25%
Environmental Science 3 15%
Immunology and Microbiology 3 15%
Agricultural and Biological Sciences 2 10%
Chemistry 2 10%
Other 2 10%
Unknown 3 15%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 July 2017.
All research outputs
#13,561,111
of 22,988,380 outputs
Outputs from AMB Express
#263
of 1,239 outputs
Outputs of similar age
#158,874
of 312,555 outputs
Outputs of similar age from AMB Express
#16
of 60 outputs
Altmetric has tracked 22,988,380 research outputs across all sources so far. This one is in the 39th percentile – i.e., 39% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,239 research outputs from this source. They receive a mean Attention Score of 2.8. This one has done well, scoring higher than 77% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 312,555 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 47th percentile – i.e., 47% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 60 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 71% of its contemporaries.