Title |
Detection of bacterial sulfatase activity through liquid- and solid-phase colony-based assays
|
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Published in |
AMB Express, July 2017
|
DOI | 10.1186/s13568-017-0449-3 |
Pubmed ID | |
Authors |
Hey Young Yoon, Hyung Jun Kim, Soojin Jang, Jong-In Hong |
Abstract |
Bacterial arylsulfatases are crucial to biosynthesis in many microorganisms, as bacteria often utilize aryl sulfates as a source of sulfur. The bacterial sulfatases are associated with pathogenesis and are applied in many areas such as industry and agriculture. We developed an activity-based probe 1 for detection of bacterial sulfatase activity through liquid- and solid-phase colony-based assays. Probe 1 is hydrolyzed by sulfatase to generate fluorescent N-methyl isoindole, which is polymerized to form colored precipitates. These fluorescent and colorimetric properties of probe 1 induced upon treatment of sulfatases were successfully utilized for liquid-phase sulfatase activity assays for colonies and lysates of Klebsiella aerogenes, Mycobacterium avium and Mycobacterium smegmatis. In addition, probe 1 allowed solid-phase colony-based assays of K. aerogenes through the formation of insoluble colored precipitates, thus enabling accurate staining of target colonies under heterogeneous conditions. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Australia | 1 | 25% |
Unknown | 3 | 75% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 4 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 20 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 5 | 25% |
Student > Master | 5 | 25% |
Student > Ph. D. Student | 2 | 10% |
Student > Doctoral Student | 2 | 10% |
Student > Bachelor | 1 | 5% |
Other | 3 | 15% |
Unknown | 2 | 10% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 5 | 25% |
Environmental Science | 3 | 15% |
Immunology and Microbiology | 3 | 15% |
Agricultural and Biological Sciences | 2 | 10% |
Chemistry | 2 | 10% |
Other | 2 | 10% |
Unknown | 3 | 15% |