Title |
Topical Delivery of Coumestrol from Lipid Nanoemulsions Thickened with Hydroxyethylcellulose for Antiherpes Treatment
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Published in |
AAPS PharmSciTech, July 2017
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DOI | 10.1208/s12249-017-0828-8 |
Pubmed ID | |
Authors |
Débora Fretes Argenta, Juliana Bidone, Letícia Scherer Koester, Valquíria Link Bassani, Cláudia Maria Oliveira Simões, Helder Ferreira Teixeira |
Abstract |
We have recently shown that coumestrol, an isoflavonoid-like compound naturally occurring in soybeans, alfafa, and red clover, inhibited Herpes Simplex Virus types 1 (HSV-1) and 2 (HSV-2) replication. In this study, we designed coumestrol formulations in an attempt to enable its topical delivery to mucosa tissues. Physicochemical and microscopic examinations suggested that coumestrol was efficiently incorporated in positively-charged nanoemulsions dispersed in a hydroxyethylcellulose gel. The higher coumestrol flux through excised porcine esophageal mucosa was detected from nanoemulsions composed by a fluid phospholipid (dioleylphosphocholine, DOPC) in comparison with that of a rigid one (distearoylphosphocholine, DSPC) in two mucosa conditions (intact and injured). Such results were supported by confocal fluorescence images. Furthermore, the low IC50 values demonstrated an increasement in the antiviral inhibition against HSV-1 and HSV-2 after incorporation of coumestrol into nanoemulsions containing DOPC. Overall, coumestrol-loaded nanoemulsions proved to be beneficial for herpes simplex treatment. |
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Unknown | 1 | 100% |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
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Unknown | 30 | 100% |
Demographic breakdown
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Student > Ph. D. Student | 5 | 17% |
Student > Master | 5 | 17% |
Student > Postgraduate | 4 | 13% |
Student > Doctoral Student | 3 | 10% |
Professor | 2 | 7% |
Other | 1 | 3% |
Unknown | 10 | 33% |
Readers by discipline | Count | As % |
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Pharmacology, Toxicology and Pharmaceutical Science | 7 | 23% |
Biochemistry, Genetics and Molecular Biology | 4 | 13% |
Chemistry | 3 | 10% |
Agricultural and Biological Sciences | 2 | 7% |
Computer Science | 1 | 3% |
Other | 1 | 3% |
Unknown | 12 | 40% |