| Title |
Development of a new method based on unmodified gold nanoparticles and peptide nucleic acids for detecting bovine viral diarrhea virus-RNA
|
|---|---|
| Published in |
AMB Express, June 2017
|
| DOI | 10.1186/s13568-017-0432-z |
| Pubmed ID | |
| Authors |
Maryam Askaravi, Seyedeh Elham Rezatofighi, Saadat Rastegarzadeh, Masoud Reza Seifi Abad Shapouri |
| Abstract |
A simple colorimetric assay is presented for detecting bovine viral diarrhea virus (BVDV)-RNA based on aggregation of gold nanoparticles (AuNPs) in the presence of charge-neutral peptide nucleic acids (PNA). Free charge-neutral PNA oligomers tended to be adsorbed onto AuNPs and act as a coagulant, whereas hybridizing complementary RNA with PNA disrupted PNA-induced AuNP aggregation, and the NPs remained stable. However, non-complementary RNA did not have this effect, and PNA induced aggregation of the AuNPs that resulted in a color change of the reaction from red to blue. The label-free colorimetric assay developed was estimated to have a 10.48 ng/reaction BVDV-RNA detection limit for the visual assay and 1.05 ng/reaction BVDV-RNA using a spectrophotometer. Diagnostic sensitivity and specificity for the assay was in accordance with real-time reverse transcriptase-polymerase chain reaction (RT-PCR) and nested RT-PCR results were 98 and 100%, respectively. Absorption of the 520/620 nm ratio was linear, along with an increase in the target RNA concentration of 1.64-52.4 ng/reaction (R(2) = 0.992), which showed a linear correlation for the quantitative assay. This study established a rapid visual label and enzyme-free diagnostic assay for detecting BVDV that is applicable in any clinical laboratory. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 39 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 39 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 12 | 31% |
| Researcher | 6 | 15% |
| Student > Master | 4 | 10% |
| Student > Bachelor | 4 | 10% |
| Lecturer > Senior Lecturer | 1 | 3% |
| Other | 3 | 8% |
| Unknown | 9 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 7 | 18% |
| Veterinary Science and Veterinary Medicine | 6 | 15% |
| Chemical Engineering | 3 | 8% |
| Biochemistry, Genetics and Molecular Biology | 3 | 8% |
| Agricultural and Biological Sciences | 3 | 8% |
| Other | 6 | 15% |
| Unknown | 11 | 28% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 June 2017.
All research outputs
#21,020,571
of 23,655,983 outputs
Outputs from AMB Express
#980
of 1,255 outputs
Outputs of similar age
#276,466
of 316,503 outputs
Outputs of similar age from AMB Express
#67
of 70 outputs
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