Title |
Utilization of acid pre-treated coconut dregs as a substrate for production of detergent compatible lipase by Bacillus stratosphericus
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Published in |
AMB Express, June 2017
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DOI | 10.1186/s13568-017-0433-y |
Pubmed ID | |
Authors |
Nur Bainun Mohd Zin, Busyra Mohamad Yusof, Siti Nurbaya Oslan, Helmi Wasoh, Joo Shun Tan, Arbakariya B. Ariff, Murni Halim |
Abstract |
In recent years, many efforts have been directed to explore the methods to reduce the production costs of industrial lipase by improving the yield and the use of low-cost agricultural wastes. Coconut dregs, which is a lignocellulosic by-product from coconut oil and milk processing plants, is rich in cellulose (36%) and crude fat (9%). A newly isolated Bacillus stratosphericus has been demonstrated to perform cellulose hydrolysis on coconut dregs producing fermentable sugars. The highest extracellular lipase activity of 140 U/mL has been achieved in submerged fermentation with acid pre-treated coconut dregs. The lipase was found to be active over a wide range of temperatures and pHs. The activity of lipase can be generally increased by the presence of detergent ingredients such as Tween-80, cetyltrimethylammonium bromide, hydrogen peroxide and phosphate per sulphate. The great compatibility of lipase in commercial detergents has also underlined its potential as an additive ingredient in biodetergent formulations. |
X Demographics
Geographical breakdown
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 50 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Bachelor | 10 | 20% |
Student > Ph. D. Student | 6 | 12% |
Student > Master | 3 | 6% |
Researcher | 2 | 4% |
Student > Postgraduate | 2 | 4% |
Other | 5 | 10% |
Unknown | 22 | 44% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 8 | 16% |
Engineering | 3 | 6% |
Biochemistry, Genetics and Molecular Biology | 3 | 6% |
Environmental Science | 2 | 4% |
Nursing and Health Professions | 2 | 4% |
Other | 7 | 14% |
Unknown | 25 | 50% |