| Title |
In vitro culture conditions to study keratinocyte differentiation using the HaCaT cell line
|
|---|---|
| Published in |
Methods in Cell Science, June 2007
|
| DOI | 10.1007/s10616-007-9076-1 |
| Pubmed ID | |
| Authors |
Adeline F. Deyrieux, V. G. Wilson |
| Abstract |
In vitro models to study the process of keratinocyte differentiation have been hindered by the stringent culture requirements and limitations imposed by the inherent properties of the cells. Primary keratinocytes only have a finite life span, while transformed cell lines exhibit many phenotypic features not found in normal cells. The spontaneously immortalized HaCaT cell line has been a widely employed keratinocyte model due to its ease of propagation and near normal phenotype, but protocols for differentiation and gene delivery into HaCaT cells vary widely in the literature. Here we report culture conditions for maintaining HaCaT cells in a basal-like state, for efficient differentiation of these cells, and for delivery of transgenes by transfection or adenoviral infection. This technological report will provide guidance to a large audience of scientists interested in investigating mechanisms of differentiation and skin morphogenesis. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 423 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Spain | 3 | <1% |
| United Kingdom | 2 | <1% |
| Finland | 1 | <1% |
| Italy | 1 | <1% |
| Portugal | 1 | <1% |
| Slovenia | 1 | <1% |
| Saudi Arabia | 1 | <1% |
| China | 1 | <1% |
| United States | 1 | <1% |
| Other | 0 | 0% |
| Unknown | 411 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 95 | 22% |
| Researcher | 69 | 16% |
| Student > Master | 68 | 16% |
| Student > Bachelor | 37 | 9% |
| Student > Doctoral Student | 22 | 5% |
| Other | 44 | 10% |
| Unknown | 88 | 21% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 123 | 29% |
| Biochemistry, Genetics and Molecular Biology | 84 | 20% |
| Medicine and Dentistry | 29 | 7% |
| Pharmacology, Toxicology and Pharmaceutical Science | 24 | 6% |
| Immunology and Microbiology | 18 | 4% |
| Other | 46 | 11% |
| Unknown | 99 | 23% |
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 January 2021.
All research outputs
#7,990,112
of 25,448,590 outputs
Outputs from Methods in Cell Science
#341
of 1,026 outputs
Outputs of similar age
#26,981
of 78,505 outputs
Outputs of similar age from Methods in Cell Science
#4
of 8 outputs
Altmetric has tracked 25,448,590 research outputs across all sources so far. This one has received more attention than most of these and is in the 67th percentile.
So far Altmetric has tracked 1,026 research outputs from this source. They receive a mean Attention Score of 4.7. This one has gotten more attention than average, scoring higher than 66% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 78,505 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 64% of its contemporaries.
We're also able to compare this research output to 8 others from the same source and published within six weeks on either side of this one. This one has scored higher than 4 of them.