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In vitro culture conditions to study keratinocyte differentiation using the HaCaT cell line

Overview of attention for article published in Cytotechnology, June 2007
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Mentioned by

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2 tweeters

Citations

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77 Dimensions

Readers on

mendeley
277 Mendeley
Title
In vitro culture conditions to study keratinocyte differentiation using the HaCaT cell line
Published in
Cytotechnology, June 2007
DOI 10.1007/s10616-007-9076-1
Pubmed ID
Authors

Adeline F. Deyrieux, V. G. Wilson

Abstract

In vitro models to study the process of keratinocyte differentiation have been hindered by the stringent culture requirements and limitations imposed by the inherent properties of the cells. Primary keratinocytes only have a finite life span, while transformed cell lines exhibit many phenotypic features not found in normal cells. The spontaneously immortalized HaCaT cell line has been a widely employed keratinocyte model due to its ease of propagation and near normal phenotype, but protocols for differentiation and gene delivery into HaCaT cells vary widely in the literature. Here we report culture conditions for maintaining HaCaT cells in a basal-like state, for efficient differentiation of these cells, and for delivery of transgenes by transfection or adenoviral infection. This technological report will provide guidance to a large audience of scientists interested in investigating mechanisms of differentiation and skin morphogenesis.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 277 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Spain 3 1%
United Kingdom 2 <1%
Italy 1 <1%
Slovenia 1 <1%
France 1 <1%
Portugal 1 <1%
Finland 1 <1%
China 1 <1%
United States 1 <1%
Other 1 <1%
Unknown 264 95%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 79 29%
Researcher 55 20%
Student > Master 53 19%
Student > Bachelor 29 10%
Unspecified 21 8%
Other 40 14%
Readers by discipline Count As %
Agricultural and Biological Sciences 121 44%
Biochemistry, Genetics and Molecular Biology 52 19%
Unspecified 27 10%
Medicine and Dentistry 26 9%
Pharmacology, Toxicology and Pharmaceutical Science 13 5%
Other 38 14%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 August 2018.
All research outputs
#8,485,886
of 13,530,841 outputs
Outputs from Cytotechnology
#437
of 621 outputs
Outputs of similar age
#154,336
of 269,651 outputs
Outputs of similar age from Cytotechnology
#1
of 5 outputs
Altmetric has tracked 13,530,841 research outputs across all sources so far. This one is in the 23rd percentile – i.e., 23% of other outputs scored the same or lower than it.
So far Altmetric has tracked 621 research outputs from this source. They receive a mean Attention Score of 4.0. This one is in the 23rd percentile – i.e., 23% of its peers scored the same or lower than it.
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We're also able to compare this research output to 5 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them