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Insights into bacterial CO2 metabolism revealed by the characterization of four carbonic anhydrases in Ralstonia eutropha H16

Overview of attention for article published in AMB Express, January 2014
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About this Attention Score

  • Among the highest-scoring outputs from this source (#48 of 199)
  • Average Attention Score compared to outputs of the same age
  • Good Attention Score compared to outputs of the same age and source (73rd percentile)

Mentioned by

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1 tweeter

Citations

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33 Dimensions

Readers on

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95 Mendeley
Title
Insights into bacterial CO2 metabolism revealed by the characterization of four carbonic anhydrases in Ralstonia eutropha H16
Published in
AMB Express, January 2014
DOI 10.1186/2191-0855-4-2
Pubmed ID
Authors

Claudia S Gai, Jingnan Lu, Christopher J Brigham, Amanda C Bernardi, Anthony J Sinskey

Abstract

Carbonic anhydrase (CA) enzymes catalyze the interconversion of CO2 and bicarbonate. These enzymes play important roles in cellular metabolism, CO2 transport, ion transport, and internal pH regulation. Understanding the metabolic role of CAs in the chemolithoautotropic bacterium Ralstonia eutropha is important for the development of high performance fermentation processes based on the bacterium's capability to fix carbon using the Calvin-Benson-Bassham (CBB) cycle. Analysis of the R. eutropha H16 genome sequence revealed the presence of four CA genes: can, can2, caa and cag. We evaluated the importance of each of the CAs in the metabolism of R. eutropha by examination of growth and enzyme activity in gene deletion, complementation, and overexpression strains. All four purified CAs were capable of performing the interconversion of CO2 and HCO3-, although the equilibrium towards the formation of CO2 or HCO3- differs with each CA. Deletion of can, encoding a β-CA, affected the growth of R. eutropha; however the growth defect could be compensated by adding CO2 to the culture. Deletion of the caa, encoding an α-CA, had the strongest deleterious influence on cell growth. Strains with deletion or overexpression of can2 or cag genes exhibited similar behavior to wild type under most of the conditions tested. In this work, Caa was studied in greater detail using microscopy and complementation experiments, which helped confirm its periplasmic localization and determine its importance for robust growth of R. eutropha. A hypothesis for the coordinated role of these four enzymes in the metabolism of R. eutropha is proposed.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 95 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Portugal 1 1%
Germany 1 1%
France 1 1%
Austria 1 1%
South Africa 1 1%
United Kingdom 1 1%
Spain 1 1%
United States 1 1%
Unknown 87 92%

Demographic breakdown

Readers by professional status Count As %
Researcher 23 24%
Student > Ph. D. Student 22 23%
Student > Master 17 18%
Student > Bachelor 6 6%
Professor > Associate Professor 5 5%
Other 13 14%
Unknown 9 9%
Readers by discipline Count As %
Agricultural and Biological Sciences 29 31%
Biochemistry, Genetics and Molecular Biology 25 26%
Engineering 6 6%
Chemistry 6 6%
Chemical Engineering 3 3%
Other 11 12%
Unknown 15 16%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 January 2014.
All research outputs
#2,302,505
of 4,507,509 outputs
Outputs from AMB Express
#48
of 199 outputs
Outputs of similar age
#59,129
of 122,934 outputs
Outputs of similar age from AMB Express
#1
of 15 outputs
Altmetric has tracked 4,507,509 research outputs across all sources so far. This one is in the 35th percentile – i.e., 35% of other outputs scored the same or lower than it.
So far Altmetric has tracked 199 research outputs from this source. They receive a mean Attention Score of 1.2. This one has gotten more attention than average, scoring higher than 65% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 122,934 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 37th percentile – i.e., 37% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 15 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 73% of its contemporaries.