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Autolysis of Pichia pastoris induced by cold

Overview of attention for article published in AMB Express, May 2017
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Mentioned by

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2 tweeters

Citations

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1 Dimensions

Readers on

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29 Mendeley
Title
Autolysis of Pichia pastoris induced by cold
Published in
AMB Express, May 2017
DOI 10.1186/s13568-017-0397-y
Pubmed ID
Authors

Yaneth Bartolo-Aguilar, Luc Dendooven, Cipriano Chávez-Cabrera, Luis B. Flores-Cotera, María E. Hidalgo-Lara, Lourdes Villa-Tanaca, Rodolfo Marsch

Abstract

The production of recombinant biopharmaceutical proteins is a multi-billion dollar market. Protein recovery represents a major part of the production costs. Pichia pastoris is one of the microbial systems most used for the production of heterologous proteins. The use of a cold-induced promoter to express lytic enzymes in the yeast after the growth stage could reduce protein recovery costs. This study shows that a cold-shock can be applied to induce lysis of the yeast cells. A strain of P. pastoris was constructed in which the endogenous eng gene encoding a putative endo-β-1,3-glucanase was overexpressed using the cold-shock induced promoter of the cctα gene from Saccharomyces cerevisiae. In the transgenic P. pastoris, the expression of eng increased 3.6-fold after chilling the cells from 30 to 4 °C (cold-shock stage) followed by incubation for 6 h (eng expression stage). The culture was heated to 30 °C for 6 h (ENG synthesis stage) and kept at 37 °C for 24 h (lysis stage). After this procedure the cell morphology changed, spheroplasts were obtained and cellular lysis was observed. Thus, a clone of P. pastoris was obtained, which undergoes autolysis after a cold-shock.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 29 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 29 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 11 38%
Student > Ph. D. Student 4 14%
Student > Postgraduate 3 10%
Student > Master 3 10%
Student > Bachelor 2 7%
Other 2 7%
Unknown 4 14%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 15 52%
Agricultural and Biological Sciences 8 28%
Veterinary Science and Veterinary Medicine 1 3%
Unknown 5 17%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 May 2017.
All research outputs
#5,486,490
of 10,033,718 outputs
Outputs from AMB Express
#198
of 638 outputs
Outputs of similar age
#135,848
of 264,010 outputs
Outputs of similar age from AMB Express
#27
of 68 outputs
Altmetric has tracked 10,033,718 research outputs across all sources so far. This one is in the 42nd percentile – i.e., 42% of other outputs scored the same or lower than it.
So far Altmetric has tracked 638 research outputs from this source. They receive a mean Attention Score of 2.2. This one has gotten more attention than average, scoring higher than 56% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 264,010 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 44th percentile – i.e., 44% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 68 others from the same source and published within six weeks on either side of this one. This one is in the 44th percentile – i.e., 44% of its contemporaries scored the same or lower than it.