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Autolysis of Pichia pastoris induced by cold

Overview of attention for article published in AMB Express, May 2017
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About this Attention Score

  • Good Attention Score compared to outputs of the same age (66th percentile)
  • High Attention Score compared to outputs of the same age and source (89th percentile)

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Title
Autolysis of Pichia pastoris induced by cold
Published in
AMB Express, May 2017
DOI 10.1186/s13568-017-0397-y
Pubmed ID
Authors

Yaneth Bartolo-Aguilar, Luc Dendooven, Cipriano Chávez-Cabrera, Luis B. Flores-Cotera, María E. Hidalgo-Lara, Lourdes Villa-Tanaca, Rodolfo Marsch

Abstract

The production of recombinant biopharmaceutical proteins is a multi-billion dollar market. Protein recovery represents a major part of the production costs. Pichia pastoris is one of the microbial systems most used for the production of heterologous proteins. The use of a cold-induced promoter to express lytic enzymes in the yeast after the growth stage could reduce protein recovery costs. This study shows that a cold-shock can be applied to induce lysis of the yeast cells. A strain of P. pastoris was constructed in which the endogenous eng gene encoding a putative endo-β-1,3-glucanase was overexpressed using the cold-shock induced promoter of the cctα gene from Saccharomyces cerevisiae. In the transgenic P. pastoris, the expression of eng increased 3.6-fold after chilling the cells from 30 to 4 °C (cold-shock stage) followed by incubation for 6 h (eng expression stage). The culture was heated to 30 °C for 6 h (ENG synthesis stage) and kept at 37 °C for 24 h (lysis stage). After this procedure the cell morphology changed, spheroplasts were obtained and cellular lysis was observed. Thus, a clone of P. pastoris was obtained, which undergoes autolysis after a cold-shock.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 40 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 40 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 11 28%
Student > Postgraduate 5 13%
Student > Ph. D. Student 5 13%
Student > Master 3 8%
Student > Bachelor 2 5%
Other 4 10%
Unknown 10 25%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 18 45%
Agricultural and Biological Sciences 9 23%
Chemical Engineering 1 3%
Veterinary Science and Veterinary Medicine 1 3%
Unknown 11 28%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 January 2024.
All research outputs
#7,130,579
of 25,223,158 outputs
Outputs from AMB Express
#128
of 1,317 outputs
Outputs of similar age
#103,688
of 316,225 outputs
Outputs of similar age from AMB Express
#8
of 69 outputs
Altmetric has tracked 25,223,158 research outputs across all sources so far. This one has received more attention than most of these and is in the 71st percentile.
So far Altmetric has tracked 1,317 research outputs from this source. They receive a mean Attention Score of 3.2. This one has done well, scoring higher than 89% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 316,225 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 66% of its contemporaries.
We're also able to compare this research output to 69 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 89% of its contemporaries.