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Use of UPLC-ESI-MS/MS to quantitate free amino acid concentrations in micro-samples of mammalian milk

Overview of attention for article published in SpringerPlus, November 2013
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  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (87th percentile)
  • High Attention Score compared to outputs of the same age and source (91st percentile)

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1 news outlet
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Citations

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57 Mendeley
Title
Use of UPLC-ESI-MS/MS to quantitate free amino acid concentrations in micro-samples of mammalian milk
Published in
SpringerPlus, November 2013
DOI 10.1186/2193-1801-2-622
Pubmed ID
Authors

Véronique Ferchaud Roucher, Emmanuelle Desnots, Charlotte Naël, Aurore Martin Agnoux, Marie-Cécile Alexandre-Gouabau, Dominique Darmaun, Clair-Yves Boquien

Abstract

Although free amino acids (FAA) account for a small fraction of total nitrogen in mammalian milk, they are more abundant in human milk than in most formulas, and may serve as a readily available source of amino acids for protein synthesis, as well as fulfill specific physiologic roles. We used reversed phase Ultra Performance Liquid Chromatography (UPLC) coupled to electrospray ionization tandem mass spectrometry (ESI-MS/MS) technique for FAA profiling in milks from three species (human, rat and cow) with a simple and rapid sample preparation. The derivatization procedure chosen, combined with UPLC-ESI-MS/MS allowed the quantitation of 21 FAA using labeled amino acids (Internal Standards) over a 10 min run time in micro-samples of mammalian milk (50 μL). The low limit of quantitation was 0.05 pmol/μL for most FAA with good repeatability and reproducibility (mean CV of 5.1%). Higher levels of total FAA were found in human (3032 μM) and rat milk (3460 μM) than in bovine milk (240 μM), with wide differences in the abundances of specific FAA between species. This robust analytical method could be applied to monitor FAA profile in human breast milk, and open the way to individualized adjustment of FAA content for the nutritional management of infants.

X Demographics

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The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 57 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 57 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 17 30%
Student > Master 8 14%
Student > Ph. D. Student 6 11%
Student > Bachelor 5 9%
Student > Doctoral Student 4 7%
Other 10 18%
Unknown 7 12%
Readers by discipline Count As %
Agricultural and Biological Sciences 19 33%
Chemistry 7 12%
Biochemistry, Genetics and Molecular Biology 6 11%
Nursing and Health Professions 3 5%
Medicine and Dentistry 2 4%
Other 9 16%
Unknown 11 19%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 10. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 29 May 2020.
All research outputs
#3,108,628
of 22,733,113 outputs
Outputs from SpringerPlus
#185
of 1,853 outputs
Outputs of similar age
#37,435
of 302,015 outputs
Outputs of similar age from SpringerPlus
#8
of 93 outputs
Altmetric has tracked 22,733,113 research outputs across all sources so far. Compared to these this one has done well and is in the 86th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,853 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.7. This one has done well, scoring higher than 89% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 302,015 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 87% of its contemporaries.
We're also able to compare this research output to 93 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 91% of its contemporaries.