| Title |
Antioxidant and antiapoptotic properties of melatonin restore intestinal calcium absorption altered by menadione
|
|---|---|
| Published in |
Molecular and Cellular Biochemistry, November 2013
|
| DOI | 10.1007/s11010-013-1885-2 |
| Pubmed ID | |
| Authors |
A. Carpentieri, A. Marchionatti, V. Areco, A. Perez, V. Centeno, N. Tolosa de Talamoni |
| Abstract |
The intestinal Ca²⁺ absorption is inhibited by menadione (MEN) through oxidative stress and apoptosis. The aim of this study was to elucidate whether the antioxidant and antiapoptotic properties of melatonin (MEL) could protect the gut against the oxidant MEN. For this purpose, 4-week-old chicks were divided into four groups: (1) controls, (2) treated i.p. with MEN (2.5 μmol/kg of b.w.), (3) treated i.p. with MEL (10 mg/kg of b.w.), and (4) treated with 10 mg MEL/kg of b.w after 2.5 μmol MEN/kg of b.w. Oxidative stress was assessed by determination of glutathione (GSH) and protein carbonyl contents as well as antioxidant enzyme activities. Apoptosis was assayed by the TUNEL technique, protein expression, and activity of caspase 3. The data show that MEL restores the intestinal Ca²⁺ absorption altered by MEN. In addition, MEL reversed the effects caused by MEN such as decrease in GSH levels, increase in the carbonyl content, alteration in mitochondrial membrane permeability, and enhancement of superoxide dismutase and catalase activities. Apoptosis triggered by MEN in the intestinal cells was arrested by MEL, as indicated by normalization of the mitochondrial membrane permeability, caspase 3 activity, and DNA fragmentation. In conclusion, MEL reverses the inhibition of intestinal Ca²⁺ absorption produced by MEN counteracting oxidative stress and apoptosis. These findings suggest that MEL could be a potential drug of choice for the reversal of impaired intestinal Ca²⁺ absorption in certain gut disorders that occur with oxidative stress and apoptosis. |
Mendeley readers
The data shown below were compiled from readership statistics for 29 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Spain | 1 | 3% |
| United States | 1 | 3% |
| Argentina | 1 | 3% |
| Unknown | 26 | 90% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 5 | 17% |
| Student > Doctoral Student | 5 | 17% |
| Researcher | 5 | 17% |
| Student > Bachelor | 3 | 10% |
| Professor > Associate Professor | 2 | 7% |
| Other | 5 | 17% |
| Unknown | 4 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 7 | 24% |
| Biochemistry, Genetics and Molecular Biology | 6 | 21% |
| Medicine and Dentistry | 6 | 21% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 3% |
| Immunology and Microbiology | 1 | 3% |
| Other | 3 | 10% |
| Unknown | 5 | 17% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 November 2013.
All research outputs
#17,489,487
of 25,654,806 outputs
Outputs from Molecular and Cellular Biochemistry
#1,387
of 2,456 outputs
Outputs of similar age
#140,542
of 224,251 outputs
Outputs of similar age from Molecular and Cellular Biochemistry
#14
of 30 outputs
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We're also able to compare this research output to 30 others from the same source and published within six weeks on either side of this one. This one is in the 43rd percentile – i.e., 43% of its contemporaries scored the same or lower than it.