| Title |
Extracellular expression of glucose inhibition-resistant Cellulomonas flavigena PN-120 β-glucosidase by a diploid strain of Saccharomyces cerevisiae
|
|---|---|
| Published in |
Archives of Microbiology, November 2013
|
| DOI | 10.1007/s00203-013-0935-1 |
| Pubmed ID | |
| Authors |
David J. Mendoza-Aguayo, Héctor M. Poggi-Varaldo, Jaime García-Mena, Ana C. Ramos-Valdivia, Luis M. Salgado, Mayra de la Torre-Martínez, Teresa Ponce-Noyola |
| Abstract |
The catalytic fraction of the Cellulomonas flavigena PN-120 oligomeric β-glucosidase (BGLA) was expressed both intra- and extracellularly in a recombinant diploid of Saccharomyces cerevisiae, under limited nutrient conditions. The recombinant enzyme (BGLA¹⁵) expressed in the supernatant of a rich medium showed 582 IU/L and 99.4 IU/g dry cell, with p-nitrophenyl-β-D-glucopyranoside as substrate. BGLA¹⁵ displayed activity against cello-oligosaccharides with 2-5 glucose monomers, demonstrating that the protein is not specific for cellobiose and that the oligomeric structure is not essential for β-D-1,4-bond hydrolysis. Native β-glucosidase is inhibited almost completely at 160 mM glucose, thus limiting cellobiose hydrolysis. At 200 mM glucose concentration, BGLA¹⁵ retained more than 50 % of its maximal activity, and even at 500 mM glucose concentration, more than 30 % of its activity was preserved. Due to these characteristics of BGLA¹⁵ activity, recombinant S. cerevisiae is able to utilize cellulosic materials (cello-oligosaccharides) to produce bioethanol. |
Mendeley readers
The data shown below were compiled from readership statistics for 24 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| South Africa | 1 | 4% |
| Unknown | 23 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 7 | 29% |
| Student > Ph. D. Student | 5 | 21% |
| Professor | 4 | 17% |
| Student > Bachelor | 2 | 8% |
| Student > Master | 2 | 8% |
| Other | 3 | 13% |
| Unknown | 1 | 4% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 11 | 46% |
| Biochemistry, Genetics and Molecular Biology | 5 | 21% |
| Chemistry | 2 | 8% |
| Immunology and Microbiology | 1 | 4% |
| Environmental Science | 1 | 4% |
| Other | 2 | 8% |
| Unknown | 2 | 8% |
Attention Score in Context
This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 November 2013.
All research outputs
#5,852,820
of 22,729,647 outputs
Outputs from Archives of Microbiology
#449
of 2,765 outputs
Outputs of similar age
#52,040
of 212,425 outputs
Outputs of similar age from Archives of Microbiology
#2
of 5 outputs
Altmetric has tracked 22,729,647 research outputs across all sources so far. This one has received more attention than most of these and is in the 74th percentile.
So far Altmetric has tracked 2,765 research outputs from this source. They receive a mean Attention Score of 3.7. This one has done well, scoring higher than 83% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 212,425 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 75% of its contemporaries.
We're also able to compare this research output to 5 others from the same source and published within six weeks on either side of this one. This one has scored higher than 3 of them.