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Extracellular expression of glucose inhibition-resistant Cellulomonas flavigena PN-120 β-glucosidase by a diploid strain of Saccharomyces cerevisiae

Overview of attention for article published in Archives of Microbiology, November 2013
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • Good Attention Score compared to outputs of the same age (79th percentile)

Mentioned by

blogs
1 blog

Citations

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3 Dimensions

Readers on

mendeley
24 Mendeley
Title
Extracellular expression of glucose inhibition-resistant Cellulomonas flavigena PN-120 β-glucosidase by a diploid strain of Saccharomyces cerevisiae
Published in
Archives of Microbiology, November 2013
DOI 10.1007/s00203-013-0935-1
Pubmed ID
Authors

David J. Mendoza-Aguayo, Héctor M. Poggi-Varaldo, Jaime García-Mena, Ana C. Ramos-Valdivia, Luis M. Salgado, Mayra de la Torre-Martínez, Teresa Ponce-Noyola

Abstract

The catalytic fraction of the Cellulomonas flavigena PN-120 oligomeric β-glucosidase (BGLA) was expressed both intra- and extracellularly in a recombinant diploid of Saccharomyces cerevisiae, under limited nutrient conditions. The recombinant enzyme (BGLA¹⁵) expressed in the supernatant of a rich medium showed 582 IU/L and 99.4 IU/g dry cell, with p-nitrophenyl-β-D-glucopyranoside as substrate. BGLA¹⁵ displayed activity against cello-oligosaccharides with 2-5 glucose monomers, demonstrating that the protein is not specific for cellobiose and that the oligomeric structure is not essential for β-D-1,4-bond hydrolysis. Native β-glucosidase is inhibited almost completely at 160 mM glucose, thus limiting cellobiose hydrolysis. At 200 mM glucose concentration, BGLA¹⁵ retained more than 50 % of its maximal activity, and even at 500 mM glucose concentration, more than 30 % of its activity was preserved. Due to these characteristics of BGLA¹⁵ activity, recombinant S. cerevisiae is able to utilize cellulosic materials (cello-oligosaccharides) to produce bioethanol.

Mendeley readers

The data shown below were compiled from readership statistics for 24 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
South Africa 1 4%
Unknown 23 96%

Demographic breakdown

Readers by professional status Count As %
Researcher 6 25%
Student > Ph. D. Student 5 21%
Professor 4 17%
Student > Bachelor 2 8%
Student > Master 2 8%
Other 4 17%
Unknown 1 4%
Readers by discipline Count As %
Agricultural and Biological Sciences 11 46%
Biochemistry, Genetics and Molecular Biology 5 21%
Unspecified 2 8%
Environmental Science 1 4%
Immunology and Microbiology 1 4%
Other 3 13%
Unknown 1 4%

Attention Score in Context

This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 November 2013.
All research outputs
#2,795,095
of 12,216,894 outputs
Outputs from Archives of Microbiology
#281
of 1,530 outputs
Outputs of similar age
#34,155
of 168,717 outputs
Outputs of similar age from Archives of Microbiology
#1
of 5 outputs
Altmetric has tracked 12,216,894 research outputs across all sources so far. Compared to these this one has done well and is in the 77th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,530 research outputs from this source. They receive a mean Attention Score of 3.5. This one has done well, scoring higher than 81% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 168,717 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 79% of its contemporaries.
We're also able to compare this research output to 5 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them