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Direct cadaverine production from cellobiose using beta-glucosidase displaying Escherichia coli

Overview of attention for article published in AMB Express, November 2013
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About this Attention Score

  • Among the highest-scoring outputs from this source (#45 of 199)
  • Average Attention Score compared to outputs of the same age
  • Above-average Attention Score compared to outputs of the same age and source (62nd percentile)

Mentioned by

twitter
1 tweeter

Citations

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25 Dimensions

Readers on

mendeley
23 Mendeley
Title
Direct cadaverine production from cellobiose using beta-glucosidase displaying Escherichia coli
Published in
AMB Express, November 2013
DOI 10.1186/2191-0855-3-67
Pubmed ID
Abstract

In this study, we demonstrate the one-step production of cadaverine (1,5-diaminopentane) from cellobiose using an Escherichia coli strain displaying β-glucosidase (BGL) on its cell surface. L-lysine decarboxylase (CadA) derived from E. coli and BGL from Thermobifida fusca YX (Tfu0937) fused to the anchor protein Blc from E. coli were co-expressed using E. coli as a host. The expression of CadA was confirmed by Western blotting and BGL activity on the cell surface was evaluated using pNPG as a substrate. Growth on cellobiose as the sole carbon source was also achieved. The OD600 value of the BGL and CadA co-expressing strain was 8.0 after 48 h cultivation, which is higher than that obtained by growth on glucose (5.4 after 48 h cultivation). The engineered strain produced cadaverine from cellobiose more effectively than from glucose: 6.1 mM after 48 h from 28 g/L of consumed cellobiose, vs. 3.3 mM from 20 g/L of consumed glucose.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 23 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
France 1 4%
Unknown 22 96%

Demographic breakdown

Readers by professional status Count As %
Student > Master 7 30%
Student > Ph. D. Student 7 30%
Student > Doctoral Student 2 9%
Professor 1 4%
Student > Bachelor 1 4%
Other 3 13%
Unknown 2 9%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 9 39%
Agricultural and Biological Sciences 6 26%
Chemistry 2 9%
Veterinary Science and Veterinary Medicine 1 4%
Environmental Science 1 4%
Other 2 9%
Unknown 2 9%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 November 2013.
All research outputs
#2,330,121
of 4,505,915 outputs
Outputs from AMB Express
#45
of 199 outputs
Outputs of similar age
#51,932
of 104,116 outputs
Outputs of similar age from AMB Express
#5
of 16 outputs
Altmetric has tracked 4,505,915 research outputs across all sources so far. This one is in the 33rd percentile – i.e., 33% of other outputs scored the same or lower than it.
So far Altmetric has tracked 199 research outputs from this source. They receive a mean Attention Score of 1.1. This one has gotten more attention than average, scoring higher than 73% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 104,116 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 34th percentile – i.e., 34% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 16 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 62% of its contemporaries.