| Title |
A Novel Amperometric Glutamate Biosensor Based on Glutamate Oxidase Adsorbed on Silicalite
|
|---|---|
| Published in |
Discover Nano, April 2017
|
| DOI | 10.1186/s11671-017-2026-8 |
| Pubmed ID | |
| Authors |
O. V. Soldatkina, O. O. Soldatkin, B. Ozansoy Kasap, D. Yu. Kucherenko, I. S. Kucherenko, B. Akata Kurc, S. V. Dzyadevych |
| Abstract |
In this work, we developed a new amperometric biosensor for glutamate detection using a typical method of glutamate oxidase (GlOx) immobilization via adsorption on silicalite particles. The disc platinum electrode (d = 0.4 mm) was used as the amperometric sensor. The procedure of biosensor preparation was optimized. The main parameters of modifying amperometric transducers with a silicalite layer were determined along with the procedure of GlOx adsorption on this layer. The biosensors based on GlOx adsorbed on silicalite demonstrated high sensitivity to glutamate. The linear range of detection was from 2.5 to 450 μM, and the limit of glutamate detection was 1 μM. It was shown that the proposed biosensors were characterized by good response reproducibility during hours of continuous work and operational stability for several days. The developed biosensors could be applied for determination of glutamate in real samples. |
Mendeley readers
The data shown below were compiled from readership statistics for 49 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 49 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 7 | 14% |
| Researcher | 7 | 14% |
| Student > Bachelor | 5 | 10% |
| Professor | 4 | 8% |
| Student > Doctoral Student | 3 | 6% |
| Other | 9 | 18% |
| Unknown | 14 | 29% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 9 | 18% |
| Biochemistry, Genetics and Molecular Biology | 6 | 12% |
| Engineering | 5 | 10% |
| Agricultural and Biological Sciences | 4 | 8% |
| Materials Science | 3 | 6% |
| Other | 2 | 4% |
| Unknown | 20 | 41% |