| Title |
Tracking dendritic cell migration into lymph nodes by using a novel PET probe 18F-tetrafluoroborate for sodium/iodide symporter
|
|---|---|
| Published in |
EJNMMI Research, April 2017
|
| DOI | 10.1186/s13550-017-0280-5 |
| Pubmed ID | |
| Authors |
Sang Bong Lee, Ho Won Lee, Hongje Lee, Yong Hyun Jeon, Sang-Woo Lee, Byeong-Cheol Ahn, Jaetae Lee, Shin Young Jeong |
| Abstract |
Recently, (18)F-tetrafluoroborate (TFB) was used as a substrate for the human sodium/iodide symporter (hNIS) reporter gene. This study evaluated the feasibility of performing molecular-genetic imaging by using the new radiotracer ((18)F-TFB) for the hNIS gene, to track dendritic cell (DC) migration in live mice. A murine dendritic cell line (DC2.4) co-expressing the hNIS and effluc genes (DC/NF) was established. To confirm the functional cellular expression of both effluc and NIS in the inoculated DC/NF cells by bio-medical imaging, combined bioluminescence imaging (BLI) and (18)F-TFB positron emission tomography/computed tomography (PET/CT) imaging was performed after intramuscular injection with parental DCs and DC/NF cells. For DC-tracking, parental DCs or DC/NF cells were injected in the left or right mouse footpad, respectively, and (18)F-TFB PET/CT and BLI were performed to monitor these cells in live mice. In vivo PET/CT and BLI showed a clear signal in DC/NF injection sites but not in parental DC injection sites. The signal intensity in DC/NF cells was correlated with time. In vivo (18)F-TFB PET/CT imaging showed higher radiotracer activity in the draining popliteal lymph nodes (DPLNs) in DC/NF injection sites than those in DC injection sites on day 2. BLI also showed DC/NF cell migration to the DPLNs on day 2 after the injection. Migration of DCs to the lymph nodes was successfully monitored using (18)F-TFB PET/CT imaging of the NIS gene and optical imaging of the effluc gene in live mice. These data support the feasibility of using (18)F-TFB as a substrate for hNIS reporter gene imaging to track the migration of DCs to the lymph nodes in live animals. The use of (18)F-TFB may facilitate enhanced PET imaging of the hNIS reporter gene in small animals and humans in future studies. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Science communicators (journalists, bloggers, editors) | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 32 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 32 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 6 | 19% |
| Student > Ph. D. Student | 6 | 19% |
| Student > Bachelor | 4 | 13% |
| Student > Doctoral Student | 4 | 13% |
| Student > Master | 2 | 6% |
| Other | 2 | 6% |
| Unknown | 8 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 6 | 19% |
| Pharmacology, Toxicology and Pharmaceutical Science | 4 | 13% |
| Immunology and Microbiology | 3 | 9% |
| Biochemistry, Genetics and Molecular Biology | 3 | 9% |
| Agricultural and Biological Sciences | 1 | 3% |
| Other | 4 | 13% |
| Unknown | 11 | 34% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 June 2017.
All research outputs
#14,929,039
of 22,962,258 outputs
Outputs from EJNMMI Research
#230
of 563 outputs
Outputs of similar age
#184,208
of 308,980 outputs
Outputs of similar age from EJNMMI Research
#10
of 13 outputs
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So far Altmetric has tracked 563 research outputs from this source. They receive a mean Attention Score of 2.5. This one has gotten more attention than average, scoring higher than 54% of its peers.
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We're also able to compare this research output to 13 others from the same source and published within six weeks on either side of this one. This one is in the 15th percentile – i.e., 15% of its contemporaries scored the same or lower than it.