| Title |
Cyclic enterobacterial common antigen: Potential contaminant of bacterially expressed protein preparations
|
|---|---|
| Published in |
Journal of Biomolecular NMR, June 2004
|
| DOI | 10.1023/b:jnmr.0000019252.65073.24 |
| Pubmed ID | |
| Authors |
Paul J.A. Erbel, Ronald Seidel, Scott E. Macintosh, Lisa N. Gentile, Juan C. Amor, Richard A. Kahn, James H. Prestegard, Lawrence P. McIntosh, Kevin H. Gardner |
| Abstract |
We have previously reported the identification of the cyclic enterobacterial common antigen (ECA(CYC)) polysaccharide in E. coli strains commonly used for heterologous protein expression (PJA Erbel et al., J. Bacteriol. 185 (2003): 1995). Following this initial report, interactions among several NMR groups established that characteristic N -acetyl signals of ECA(CYC) have been observed in (15)N-(1)H HSQC spectra of samples of various bacterially-expressed proteins suggesting that this water-soluble carbohydrate is a common contaminant. We provide NMR spectroscopic tools to recognize ECA(CYC) in protein samples, as well as several methods to remove this contaminant. Early recognition of ECA-based NMR signals will prevent time-consuming analyses of this copurifying carbohydrate. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 22 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Netherlands | 1 | 5% |
| Unknown | 21 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 8 | 36% |
| Student > Ph. D. Student | 7 | 32% |
| Professor | 4 | 18% |
| Professor > Associate Professor | 2 | 9% |
| Unknown | 1 | 5% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 7 | 32% |
| Chemistry | 7 | 32% |
| Physics and Astronomy | 2 | 9% |
| Biochemistry, Genetics and Molecular Biology | 2 | 9% |
| Unknown | 4 | 18% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 October 2013.
All research outputs
#17,285,668
of 25,374,647 outputs
Outputs from Journal of Biomolecular NMR
#380
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#56,394
of 62,304 outputs
Outputs of similar age from Journal of Biomolecular NMR
#15
of 16 outputs
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