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Cyclic enterobacterial common antigen: Potential contaminant of bacterially expressed protein preparations

Overview of attention for article published in Journal of Biomolecular NMR, June 2004
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1 tweeter

Citations

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Readers on

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19 Mendeley
Title
Cyclic enterobacterial common antigen: Potential contaminant of bacterially expressed protein preparations
Published in
Journal of Biomolecular NMR, June 2004
DOI 10.1023/b:jnmr.0000019252.65073.24
Pubmed ID
Authors

Paul J.A. Erbel, Ronald Seidel, Scott E. Macintosh, Lisa N. Gentile, Juan C. Amor, Richard A. Kahn, James H. Prestegard, Lawrence P. McIntosh, Kevin H. Gardner

Abstract

We have previously reported the identification of the cyclic enterobacterial common antigen (ECA(CYC)) polysaccharide in E. coli strains commonly used for heterologous protein expression (PJA Erbel et al., J. Bacteriol. 185 (2003): 1995). Following this initial report, interactions among several NMR groups established that characteristic N -acetyl signals of ECA(CYC) have been observed in (15)N-(1)H HSQC spectra of samples of various bacterially-expressed proteins suggesting that this water-soluble carbohydrate is a common contaminant. We provide NMR spectroscopic tools to recognize ECA(CYC) in protein samples, as well as several methods to remove this contaminant. Early recognition of ECA-based NMR signals will prevent time-consuming analyses of this copurifying carbohydrate.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 19 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Netherlands 1 5%
Unknown 18 95%

Demographic breakdown

Readers by professional status Count As %
Researcher 7 37%
Student > Ph. D. Student 6 32%
Unspecified 3 16%
Professor > Associate Professor 2 11%
Professor 1 5%
Other 0 0%
Readers by discipline Count As %
Chemistry 6 32%
Agricultural and Biological Sciences 6 32%
Unspecified 5 26%
Physics and Astronomy 2 11%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 October 2013.
All research outputs
#2,584,134
of 4,870,132 outputs
Outputs from Journal of Biomolecular NMR
#135
of 265 outputs
Outputs of similar age
#51,935
of 104,492 outputs
Outputs of similar age from Journal of Biomolecular NMR
#1
of 8 outputs
Altmetric has tracked 4,870,132 research outputs across all sources so far. This one is in the 33rd percentile – i.e., 33% of other outputs scored the same or lower than it.
So far Altmetric has tracked 265 research outputs from this source. They receive a mean Attention Score of 1.9. This one is in the 33rd percentile – i.e., 33% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 104,492 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 36th percentile – i.e., 36% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 8 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them