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Comparing cellular performance of Yarrowia lipolytica during growth on glucose and glycerol in submerged cultivations

Overview of attention for article published in AMB Express, October 2013
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Title
Comparing cellular performance of Yarrowia lipolytica during growth on glucose and glycerol in submerged cultivations
Published in
AMB Express, October 2013
DOI 10.1186/2191-0855-3-58
Pubmed ID
Authors

Mhairi Workman, Philippe Holt, Jette Thykaer

Abstract

Yarrowia lipolytica is an attractive host for sustainable bioprocesses due to its ability to utilize a variety of carbon substrates and convert them to a range of different product types (including lipids, organic acids and polyols) under specific conditions. Despite an increasing number of applications for this yeast, relatively few studies have focused on uptake and metabolism of carbon sources, and the metabolic basis for carbon flow to the different products. The focus of this work was quantification of the cellular performance of Y. lipolytica during growth on glycerol, glucose or a mixture of the two. Carbon substrate uptake rate, growth rate, oxygen utilisation (requirement and uptake rate) and polyol yields were estimated in batch cultivations at 1 litre scale. When glucose was used as the sole carbon and energy source, the growth rate was 0.24 h-1 and biomass and CO2 were the only products. Growth on glycerol proceeded at approximately 0.30 h-1, and the substrate uptake rate was 0.02 mol L-1 h-1 regardless of the starting glycerol concentration (10, 20 or 45 g L-1). Utilisation of glycerol was accompanied by higher oxygen uptake rates compared to glucose growth, indicating import of glycerol occurred initially via phosphorylation of glycerol into glycerol-3-phosphate. Based on these results it could be speculated that once oxygen limitation was reached, additional production of NADH created imbalance in the cofactor pools and the polyol formation observed could be a result of cofactor recycling to restore the balance in metabolism.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 220 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Denmark 3 1%
France 2 <1%
Brazil 1 <1%
Sweden 1 <1%
United States 1 <1%
Unknown 212 96%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 60 27%
Student > Ph. D. Student 34 15%
Student > Master 28 13%
Researcher 20 9%
Student > Doctoral Student 12 5%
Other 17 8%
Unknown 49 22%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 63 29%
Agricultural and Biological Sciences 61 28%
Engineering 23 10%
Chemical Engineering 9 4%
Chemistry 5 2%
Other 7 3%
Unknown 52 24%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 October 2013.
All research outputs
#22,759,452
of 25,374,647 outputs
Outputs from AMB Express
#1,038
of 1,325 outputs
Outputs of similar age
#195,310
of 220,241 outputs
Outputs of similar age from AMB Express
#9
of 14 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,325 research outputs from this source. They receive a mean Attention Score of 3.2. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 14 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.