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An oxidoreductase from ‘Alphonso’ mango catalyzing biosynthesis of furaneol and reduction of reactive carbonyls

Overview of attention for article published in SpringerPlus, October 2013
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  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (81st percentile)
  • High Attention Score compared to outputs of the same age and source (89th percentile)

Mentioned by

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2 X users
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1 patent
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1 Facebook page
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1 Wikipedia page

Citations

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13 Dimensions

Readers on

mendeley
23 Mendeley
Title
An oxidoreductase from ‘Alphonso’ mango catalyzing biosynthesis of furaneol and reduction of reactive carbonyls
Published in
SpringerPlus, October 2013
DOI 10.1186/2193-1801-2-494
Pubmed ID
Authors

Ram Kulkarni, Hemangi Chidley, Ashish Deshpande, Axel Schmidt, Keshav Pujari, Ashok Giri, Jonathan Gershenzon, Vidya Gupta

Abstract

Two furanones, furaneol (4-hydroxy-2,5-dimethyl-3(2H)-furanone) and mesifuran (2,5-dimethyl-4-methoxy-3(2H)-furanone), are important constituents of flavor of the Alphonso cultivar of mango (Mangifera indica). To get insights into the biosynthesis of these furanones, we isolated an enone oxidoreductase gene from the Alphonso mango. It has high sequence similarity to an alkenal/one oxidoreductase from cucumber (79% identity) and enone oxidoreductases from tomato (73% identity) and strawberry (72% identity). The complete open reading frame was expressed in E. coli and the (his)6-tagged recombinant protein was purified by affinity chromatography. The purified protein assayed with NADH as a reducing agent converted D-fructose-1,6-diphosphate into furaneol, the immediate precursor of mesifuran. The enzyme was also able to convert two highly reactive carbonyls, 3-buten-2-one and 1-penten-3-one, produced by lipid peroxidation in plants, into their saturated derivatives. Expression profiling in various ripening stages of Alphonso fruits depicted an expression maxima at 10 days after harvest stage, shortly before the appearance of the maximum amount of furanones (completely ripe stage, 15 days after harvest). Although no furanones were detected at the 0 day after harvest stage, significant expression of this gene was detected in the fruits at this stage. Overall, the results suggest that this oxidoreductase plays important roles in Alphonso mango fruits.

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X Demographics

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 23 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Chile 1 4%
Unknown 22 96%

Demographic breakdown

Readers by professional status Count As %
Researcher 6 26%
Student > Bachelor 4 17%
Student > Master 4 17%
Professor > Associate Professor 2 9%
Student > Ph. D. Student 1 4%
Other 1 4%
Unknown 5 22%
Readers by discipline Count As %
Agricultural and Biological Sciences 8 35%
Biochemistry, Genetics and Molecular Biology 5 22%
Chemistry 2 9%
Immunology and Microbiology 1 4%
Medicine and Dentistry 1 4%
Other 1 4%
Unknown 5 22%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 8. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 November 2017.
All research outputs
#4,171,960
of 23,298,349 outputs
Outputs from SpringerPlus
#252
of 1,854 outputs
Outputs of similar age
#38,054
of 208,426 outputs
Outputs of similar age from SpringerPlus
#12
of 106 outputs
Altmetric has tracked 23,298,349 research outputs across all sources so far. Compared to these this one has done well and is in the 82nd percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,854 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.8. This one has done well, scoring higher than 86% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 208,426 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 81% of its contemporaries.
We're also able to compare this research output to 106 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 89% of its contemporaries.