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Reprogrammed mouse astrocytes retain a “memory” of tissue origin and possess more tendencies for neuronal differentiation than reprogrammed mouse embryonic fibroblasts

Overview of attention for article published in Protein & Cell, March 2011
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Title
Reprogrammed mouse astrocytes retain a “memory” of tissue origin and possess more tendencies for neuronal differentiation than reprogrammed mouse embryonic fibroblasts
Published in
Protein & Cell, March 2011
DOI 10.1007/s13238-011-1012-7
Pubmed ID
Authors

Changhai Tian, Yongxiang Wang, Lijun Sun, Kangmu Ma, Jialin C. Zheng

Abstract

Direct reprogramming of a variety of somatic cells with the transcription factors Oct4 (also called Pou5f1), Sox2 with either Klf4 and Myc or Lin28 and Nanog generates the induced pluripotent stem cells (iPSCs) with marker similarity to embryonic stem cells. However, the difference between iPSCs derived from different origins is unclear. In this study, we hypothesized that reprogrammed cells retain a "memory" of their origins and possess additional potential of related tissue differentiation. We reprogrammed primary mouse astrocytes via ectopic retroviral expression of OCT3/4, Sox2, Klf4 and Myc and found the iPSCs from mouse astrocytes expressed stem cell markers and formed teratomas in SCID mice containing derivatives of all three germ layers similar to mouse embryonic stem cells besides semblable morphologies. To test our hypothesis, we compared embryonic bodies (EBs) formation and neuronal differentiation between iPSCs from mouse embryonic fibroblasts (MEFsiPSCs) and iPSCs from mouse astrocytes (mAsiPSCs). We found that mAsiPSCs grew slower and possessed more potential for neuronal differentiation compared to MEFsiPSCs. Our results suggest that mAsiPSCs retain a "memory" of the central nervous system, which confers additional potential upon neuronal differentiation.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 29 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
France 1 3%
Australia 1 3%
Brazil 1 3%
Japan 1 3%
Unknown 25 86%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 14 48%
Researcher 6 21%
Unspecified 2 7%
Student > Postgraduate 2 7%
Other 1 3%
Other 4 14%
Readers by discipline Count As %
Agricultural and Biological Sciences 13 45%
Neuroscience 5 17%
Unspecified 4 14%
Biochemistry, Genetics and Molecular Biology 3 10%
Medicine and Dentistry 2 7%
Other 2 7%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 June 2011.
All research outputs
#11,581,774
of 13,035,139 outputs
Outputs from Protein & Cell
#338
of 423 outputs
Outputs of similar age
#11,096,235
of 12,437,442 outputs
Outputs of similar age from Protein & Cell
#315
of 397 outputs
Altmetric has tracked 13,035,139 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 423 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 11.0. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 397 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.