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Osmotic stress in colony and planktonic cells of Pseudomonas putida mt-2 revealed significant differences in adaptive response mechanisms

Overview of attention for article published in AMB Express, March 2017
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  • Above-average Attention Score compared to outputs of the same age and source (64th percentile)

Mentioned by

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2 tweeters

Citations

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9 Dimensions

Readers on

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25 Mendeley
Title
Osmotic stress in colony and planktonic cells of Pseudomonas putida mt-2 revealed significant differences in adaptive response mechanisms
Published in
AMB Express, March 2017
DOI 10.1186/s13568-017-0371-8
Pubmed ID
Authors

Nancy Hachicho, Astrid Birnbaum, Hermann J. Heipieper

Abstract

Planktonic cells and those grown on surfaces (or as colony biofilm) are known to show significant differences regarding growth behavior, cell physiology, gene expression and stress tolerance. In order to compare stress behavior of different growth forms, shake cultures for planktonic growth and agar plate cultivation for colony growth, were carried out with the well investigated model organism, Pseudomonas putida mt-2. Cells were exposed to sodium chloride to cause osmotic stress as one main environmental stressor bacteria have to cope with when growing in soil. Planktonic cells were more tolerant with a complete inhibition of growth at 0.7 M NaCl, compared to 0.5 M for agar-grown cells. Cell surface hydrophobicity, measured as water contact angles, was significantly higher for agar-grown cells (92°) than for planktonic cells (40°), and increased in the presence of NaCl. Agar-grown cells also showed a significantly higher degree of saturation of membrane fatty acids that increased in the presence of NaCl. These results demonstrate that planktonic and colony grown bacteria show different responses when confronted with osmotic stress suggesting that the tolerance and adaptive mechanisms are dependent on the environmental conditions as well as the initial physiological state.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 25 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 25 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 8 32%
Student > Ph. D. Student 4 16%
Student > Master 3 12%
Researcher 2 8%
Student > Doctoral Student 1 4%
Other 2 8%
Unknown 5 20%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 7 28%
Agricultural and Biological Sciences 6 24%
Environmental Science 1 4%
Unspecified 1 4%
Nursing and Health Professions 1 4%
Other 3 12%
Unknown 6 24%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 March 2017.
All research outputs
#4,740,868
of 9,210,205 outputs
Outputs from AMB Express
#182
of 598 outputs
Outputs of similar age
#135,287
of 254,513 outputs
Outputs of similar age from AMB Express
#15
of 57 outputs
Altmetric has tracked 9,210,205 research outputs across all sources so far. This one is in the 45th percentile – i.e., 45% of other outputs scored the same or lower than it.
So far Altmetric has tracked 598 research outputs from this source. They receive a mean Attention Score of 2.2. This one has gotten more attention than average, scoring higher than 56% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 254,513 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 42nd percentile – i.e., 42% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 57 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 64% of its contemporaries.