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Designing novel construction for cell surface display of protein E on Escherichia coli using non-classical pathway based on Lpp-OmpA

Overview of attention for article published in AMB Express, February 2017
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About this Attention Score

  • Above-average Attention Score compared to outputs of the same age (61st percentile)
  • Good Attention Score compared to outputs of the same age and source (75th percentile)

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Title
Designing novel construction for cell surface display of protein E on Escherichia coli using non-classical pathway based on Lpp-OmpA
Published in
AMB Express, February 2017
DOI 10.1186/s13568-017-0350-0
Pubmed ID
Authors

Meisam Jeiranikhameneh, Mohamad Reza Razavi, Shiva Irani, Seyed Davar Siadat, Mana Oloomi

Abstract

Today, transference of recombinant protein on the outer surface of bacteria is deemed as a valuable process for various applications in biotechnology including preparation of vaccines. In this study, Lpp-OmpA structure was used to present outer membrane protein E of Haemophilus influenzae on E. coli outer membrane. Also, a structure was designed according to Lpp-OmpA based on non-classical secretion pathway using bioinformatics software such as MEMSAT-SVM, ScrotumP and SignalP where it lacked any signal peptide at its N-terminal. Potential of this structure in the presentation of protein E on the surface of E. coli through non-classical pathway was indicated by western blotting, SDS page and fluorescent microscopy techniques, similarly its effectiveness was compared with Lpp-OmpA system. The results of the current study showed that the new structure had higher efficiency than Lpp-OmpA, and it could transport protein E on outer membrane well. This study is the first report in the presentation of H. influenzae PE onto the surface of E. coli by Lpp-OmpA, and the structure originated from Lpp-OmpA, according to the non-classical secretion pathway. Our results suggest that non-classical secretion pathway may be exploited as a new secretory pathway on the outer surface of the cell for recombinant proteins.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 65 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 65 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 14 22%
Researcher 6 9%
Student > Master 6 9%
Student > Bachelor 4 6%
Other 4 6%
Other 13 20%
Unknown 18 28%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 23 35%
Agricultural and Biological Sciences 7 11%
Nursing and Health Professions 3 5%
Immunology and Microbiology 2 3%
Chemistry 2 3%
Other 9 14%
Unknown 19 29%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 January 2022.
All research outputs
#7,235,782
of 22,867,327 outputs
Outputs from AMB Express
#156
of 1,235 outputs
Outputs of similar age
#117,331
of 310,493 outputs
Outputs of similar age from AMB Express
#13
of 56 outputs
Altmetric has tracked 22,867,327 research outputs across all sources so far. This one has received more attention than most of these and is in the 67th percentile.
So far Altmetric has tracked 1,235 research outputs from this source. They receive a mean Attention Score of 2.8. This one has done well, scoring higher than 85% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 310,493 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 61% of its contemporaries.
We're also able to compare this research output to 56 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 75% of its contemporaries.