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Loop-mediated isothermal amplification of Neisseria gonorrhoeae porA pseudogene: a rapid and reliable method to detect gonorrhea

Overview of attention for article published in AMB Express, February 2017
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • Good Attention Score compared to outputs of the same age (73rd percentile)
  • High Attention Score compared to outputs of the same age and source (92nd percentile)

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2 X users
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2 patents

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34 Mendeley
Title
Loop-mediated isothermal amplification of Neisseria gonorrhoeae porA pseudogene: a rapid and reliable method to detect gonorrhea
Published in
AMB Express, February 2017
DOI 10.1186/s13568-017-0349-6
Pubmed ID
Authors

Mei-Ling Liu, Yong Xia, Xing-Zhong Wu, Jian-Qiong Huang, Xu-Guang Guo

Abstract

Gonorrhea is a sexually transmitted disease caused by the bacterium Neisseria gonorrhoeae. Rapid detection is crucial for effective prevention and treatment. This study developed and tested a low-cost effective method for detecting N. gonorrhoeae, especially in developing countries. DNA from a N. gonorrhoeae standard strain, as well as from 26 genital secretion samples of gonorrhea patients, were isolated and used for loop-mediated isothermal amplification (LAMP) assay, which was conducted using either an automatic real-time PCR analyzer or a water bath. The amplified porA pseudogene sequence was compared with the NCBI database and the LAMP results were compared with that of the traditional culture method for its sensitivity and specificity. LAMP was able to detect Neisseria DNA at a concentration as low as 1 pg/µL (1 × 10(3) CFU/mL cells). The LAMP assay results obtained using an automatic real-time PCR analyzer was similar to that of the water bath. Relative to traditional culture, the sensitivity and specificity of the LAMP assay were 94.7 and 85.7%, respectively. LAMP was sensitive and reliable for detecting the porA gene of N. gonorrhoeae. It could be used as a rapid, low cost, and effective method for detecting N. gonorrhoeae.

X Demographics

X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 34 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 34 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 7 21%
Student > Doctoral Student 3 9%
Student > Master 2 6%
Student > Postgraduate 2 6%
Researcher 2 6%
Other 5 15%
Unknown 13 38%
Readers by discipline Count As %
Agricultural and Biological Sciences 6 18%
Biochemistry, Genetics and Molecular Biology 5 15%
Medicine and Dentistry 4 12%
Engineering 3 9%
Immunology and Microbiology 2 6%
Other 2 6%
Unknown 12 35%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 February 2024.
All research outputs
#5,143,910
of 25,366,663 outputs
Outputs from AMB Express
#100
of 1,322 outputs
Outputs of similar age
#83,993
of 317,735 outputs
Outputs of similar age from AMB Express
#5
of 55 outputs
Altmetric has tracked 25,366,663 research outputs across all sources so far. Compared to these this one has done well and is in the 79th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,322 research outputs from this source. They receive a mean Attention Score of 3.2. This one has done particularly well, scoring higher than 92% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 317,735 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 73% of its contemporaries.
We're also able to compare this research output to 55 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 92% of its contemporaries.