| Title |
A simple, portable, electrochemical biosensor to screen shellfish for Vibrio parahaemolyticus
|
|---|---|
| Published in |
AMB Express, February 2017
|
| DOI | 10.1186/s13568-017-0339-8 |
| Pubmed ID | |
| Authors |
Noordiana Nordin, Nor Azah Yusof, Jaafar Abdullah, Son Radu, Roozbeh Hushiarian |
| Abstract |
An earlier electrochemical mechanism of DNA detection was adapted and specified for the detection of Vibrio parahaemolyticus in real samples. The reader, based on a screen printed carbon electrode, was modified with polylactide-stabilized gold nanoparticles and methylene blue was employed as the redox indicator. Detection was assessed using a microprocessor to measure current response under controlled potential. The fabricated sensor was able to specifically distinguish complementary, non-complementary and mismatched oligonucleotides. DNA was measured in the range of 2.0 × 10(-8)-2.0 × 10(-13) M with a detection limit of 2.16 pM. The relative standard deviation for 6 replications of differential pulse voltammetry (DPV) measurement of 0.2 µM complementary DNA was 4.33%. Additionally, cross-reactivity studies against various other food-borne pathogens showed a reliably sensitive detection of the target pathogen. Successful identification of Vibrio parahaemolyticus (spiked and unspiked) in fresh cockles, combined with its simplicity and portability demonstrate the potential of the device as a practical screening tool. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 63 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 63 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 8 | 13% |
| Researcher | 7 | 11% |
| Student > Master | 7 | 11% |
| Student > Bachelor | 6 | 10% |
| Lecturer | 4 | 6% |
| Other | 13 | 21% |
| Unknown | 18 | 29% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 8 | 13% |
| Chemistry | 7 | 11% |
| Agricultural and Biological Sciences | 6 | 10% |
| Engineering | 5 | 8% |
| Chemical Engineering | 3 | 5% |
| Other | 7 | 11% |
| Unknown | 27 | 43% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 February 2017.
All research outputs
#20,701,376
of 23,301,510 outputs
Outputs from AMB Express
#979
of 1,251 outputs
Outputs of similar age
#387,015
of 456,149 outputs
Outputs of similar age from AMB Express
#63
of 64 outputs
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