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Expression, purification and functionality of bioactive recombinant human vascular endothelial growth factor VEGF165 in E. coli

Overview of attention for article published in AMB Express, February 2017
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75 Mendeley
Title
Expression, purification and functionality of bioactive recombinant human vascular endothelial growth factor VEGF165 in E. coli
Published in
AMB Express, February 2017
DOI 10.1186/s13568-016-0300-2
Pubmed ID
Authors

Awatef Taktak-BenAmar, Maram Morjen, Hazem Ben Mabrouk, Rania Abdelmaksoud-Dammak, Mohamed Guerfali, Najla Fourati-Masmoudi, Naziha Marrakchi, Ali Gargouri

Abstract

Vascular endothelial growth factor (VEGF) is associated with tumour growth and metastasis. Because VEGF is the major player in both angiogenesis and vascular permeability and the most explored factor in angio-inhibitory therapies, many expression procedures have been developed to produce functional VEGF165 in convenient yield. In this study, recombinant human VEGF165 was cloned and expressed in Escherichia coli (BL21)-DE3 cells and large scale production was performed by fermentation. A high yield of active soluble protein was obtained after protein extraction employing both lysozyme and sonication treatment. Inclusion bodies were also isolated from the cell lysate and subjected to a simple protocol of solubilisation and refolding. Single-step purification was performed using nickel affinity chromatography and the purified proteins were able to recognize monoclonal Anti-poly-His antibody. The biological activity of the VEGF165 was successfully tested using the Chicken chorioallantoic membrane assay, wound-healing migration and proliferation assay on human umbilical vein endothelial cells (HUVEC).

X Demographics

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The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 75 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 75 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 12 16%
Unspecified 11 15%
Researcher 11 15%
Student > Ph. D. Student 5 7%
Student > Master 4 5%
Other 8 11%
Unknown 24 32%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 20 27%
Unspecified 11 15%
Agricultural and Biological Sciences 3 4%
Medicine and Dentistry 2 3%
Chemistry 2 3%
Other 10 13%
Unknown 27 36%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 February 2017.
All research outputs
#15,442,314
of 22,952,268 outputs
Outputs from AMB Express
#445
of 1,237 outputs
Outputs of similar age
#256,500
of 420,377 outputs
Outputs of similar age from AMB Express
#23
of 60 outputs
Altmetric has tracked 22,952,268 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,237 research outputs from this source. They receive a mean Attention Score of 2.8. This one is in the 40th percentile – i.e., 40% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 420,377 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 30th percentile – i.e., 30% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 60 others from the same source and published within six weeks on either side of this one. This one is in the 5th percentile – i.e., 5% of its contemporaries scored the same or lower than it.