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Deletion of Nlrp3 protects from inflammation-induced skeletal muscle atrophy

Overview of attention for article published in Intensive Care Medicine Experimental, January 2017
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Title
Deletion of Nlrp3 protects from inflammation-induced skeletal muscle atrophy
Published in
Intensive Care Medicine Experimental, January 2017
DOI 10.1186/s40635-016-0115-0
Pubmed ID
Authors

Nora Huang, Melanie Kny, Fabian Riediger, Katharina Busch, Sibylle Schmidt, Friedrich C. Luft, Hortense Slevogt, Jens Fielitz

Abstract

Critically ill patients develop atrophic muscle failure, which increases morbidity and mortality. Interleukin-1β (IL-1β) is activated early in sepsis. Whether IL-1β acts directly on muscle cells and whether its inhibition prevents atrophy is unknown. We aimed to investigate if IL-1β activation via the Nlrp3 inflammasome is involved in inflammation-induced atrophy. We performed an experimental study and prospective animal trial. The effect of IL-1β on differentiated C2C12 muscle cells was investigated by analyzing gene-and-protein expression, and atrophy response. Polymicrobial sepsis was induced by cecum ligation and puncture surgery in Nlrp3 knockout and wild type mice. Skeletal muscle morphology, gene and protein expression, and atrophy markers were used to analyze the atrophy response. Immunostaining and reporter-gene assays showed that IL-1β signaling is contained and active in myocytes. Immunostaining and reporter gene assays showed that IL-1β signaling is contained and active in myocytes. IL-1β increased Il6 and atrogene gene expression resulting in myocyte atrophy. Nlrp3 knockout mice showed reduced IL-1β serum levels in sepsis. As determined by muscle morphology, organ weights, gene expression, and protein content, muscle atrophy was attenuated in septic Nlrp3 knockout mice, compared to septic wild-type mice 96 h after surgery. IL-1β directly acts on myocytes to cause atrophy in sepsis. Inhibition of IL-1β activation by targeting Nlrp3 could be useful to prevent inflammation-induced muscle failure in critically ill patients.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 74 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Russia 1 1%
Unknown 73 99%

Demographic breakdown

Readers by professional status Count As %
Researcher 14 19%
Student > Ph. D. Student 13 18%
Student > Doctoral Student 7 9%
Student > Master 7 9%
Student > Bachelor 5 7%
Other 8 11%
Unknown 20 27%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 17 23%
Medicine and Dentistry 9 12%
Nursing and Health Professions 8 11%
Agricultural and Biological Sciences 7 9%
Pharmacology, Toxicology and Pharmaceutical Science 3 4%
Other 10 14%
Unknown 20 27%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 January 2017.
All research outputs
#17,863,974
of 22,940,083 outputs
Outputs from Intensive Care Medicine Experimental
#307
of 449 outputs
Outputs of similar age
#292,068
of 418,156 outputs
Outputs of similar age from Intensive Care Medicine Experimental
#5
of 6 outputs
Altmetric has tracked 22,940,083 research outputs across all sources so far. This one is in the 19th percentile – i.e., 19% of other outputs scored the same or lower than it.
So far Altmetric has tracked 449 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 7.0. This one is in the 27th percentile – i.e., 27% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 418,156 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 26th percentile – i.e., 26% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 6 others from the same source and published within six weeks on either side of this one.