Title |
Development of a SYBR Green I real-time PCR for the detection of the orf virus
|
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Published in |
AMB Express, January 2017
|
DOI | 10.1186/s13568-016-0322-9 |
Pubmed ID | |
Authors |
Yong Wang, Kankan Yang, Caixia Bai, Dongdong Yin, Gang Li, Kezong Qi, Guijun Wang, Yongdong Li |
Abstract |
Orf is a non-systemic, ubiquitous disease of sheep and goats caused by the orf virus (ORFV). ORFV occasionally causes cutaneous lesions in humans in contact with infected animals. In the present study, a real-time PCR method was established for detection of ORFV using the fluorescent chimeric dye SYBR Green I. Specific primers were designed to target a highly conserved region of the ORFV B2L gene. This method was able to detect a minimum of 20 copies of ORFV genomic DNA. The results showed no cross-reactions with other common DNA viruses. The time required for the test was approximately 1.5 h. Clinical test samples showed that this method was faster and had a higher sensitivity than traditional PCR. In conclusion, this novel, real-time PCR-based assay provides a rapid, sensitive, and specific method for ORFV detection. This test provides improved technical support for studies regarding the clinical diagnosis and epidemiology of ORFV. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 12 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 4 | 33% |
Student > Master | 4 | 33% |
Student > Bachelor | 1 | 8% |
Other | 1 | 8% |
Researcher | 1 | 8% |
Other | 0 | 0% |
Unknown | 1 | 8% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 5 | 42% |
Veterinary Science and Veterinary Medicine | 2 | 17% |
Chemistry | 2 | 17% |
Immunology and Microbiology | 1 | 8% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 8% |
Other | 0 | 0% |
Unknown | 1 | 8% |