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Development of a SYBR Green I real-time PCR for the detection of the orf virus

Overview of attention for article published in AMB Express, January 2017
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Title
Development of a SYBR Green I real-time PCR for the detection of the orf virus
Published in
AMB Express, January 2017
DOI 10.1186/s13568-016-0322-9
Pubmed ID
Authors

Yong Wang, Kankan Yang, Caixia Bai, Dongdong Yin, Gang Li, Kezong Qi, Guijun Wang, Yongdong Li

Abstract

Orf is a non-systemic, ubiquitous disease of sheep and goats caused by the orf virus (ORFV). ORFV occasionally causes cutaneous lesions in humans in contact with infected animals. In the present study, a real-time PCR method was established for detection of ORFV using the fluorescent chimeric dye SYBR Green I. Specific primers were designed to target a highly conserved region of the ORFV B2L gene. This method was able to detect a minimum of 20 copies of ORFV genomic DNA. The results showed no cross-reactions with other common DNA viruses. The time required for the test was approximately 1.5 h. Clinical test samples showed that this method was faster and had a higher sensitivity than traditional PCR. In conclusion, this novel, real-time PCR-based assay provides a rapid, sensitive, and specific method for ORFV detection. This test provides improved technical support for studies regarding the clinical diagnosis and epidemiology of ORFV.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 12 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 4 33%
Student > Master 4 33%
Student > Bachelor 1 8%
Other 1 8%
Researcher 1 8%
Other 0 0%
Unknown 1 8%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 5 42%
Veterinary Science and Veterinary Medicine 2 17%
Chemistry 2 17%
Immunology and Microbiology 1 8%
Pharmacology, Toxicology and Pharmaceutical Science 1 8%
Other 0 0%
Unknown 1 8%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 January 2017.
All research outputs
#15,423,393
of 22,931,367 outputs
Outputs from AMB Express
#445
of 1,236 outputs
Outputs of similar age
#256,715
of 420,788 outputs
Outputs of similar age from AMB Express
#19
of 45 outputs
Altmetric has tracked 22,931,367 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,236 research outputs from this source. They receive a mean Attention Score of 2.8. This one is in the 40th percentile – i.e., 40% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 420,788 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 30th percentile – i.e., 30% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 45 others from the same source and published within six weeks on either side of this one. This one is in the 4th percentile – i.e., 4% of its contemporaries scored the same or lower than it.