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Alkane biosynthesis by Aspergillus carbonarius ITEM 5010 through heterologous expression of Synechococcus elongatus acyl-ACP/CoA reductase and aldehyde deformylating oxygenase genes

Overview of attention for article published in AMB Express, January 2017
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Title
Alkane biosynthesis by Aspergillus carbonarius ITEM 5010 through heterologous expression of Synechococcus elongatus acyl-ACP/CoA reductase and aldehyde deformylating oxygenase genes
Published in
AMB Express, January 2017
DOI 10.1186/s13568-016-0321-x
Pubmed ID
Authors

Malavika Sinha, Malavika Sinha, István Weyda, Annette Sørensen, Kenneth S. Bruno, Birgitte K. Ahring

Abstract

In this study we describe the heterologous expression of the recently identified cyanobacterial pathway for long chain alkane biosynthesis, involving the reduction of fatty acyl-ACP to fatty aldehyde and the subsequent conversion of this into alkanes, in the filamentous fungus Aspergillus carbonarius ITEM 5010. Genes originating from Synechococcus elongatus strain PCC7942, encoding acyl-ACP/CoA reductase and aldehyde deformylating oxygenase enzymes, were successfully expressed in A. carbonarius, which lead to the production of pentadecane and heptadecane, alkanes that have not been previously produced by this fungus. Titers of 0.2, 0.5 and 2.7 mg/l pentadecane and 0.8, 1.6 and 10.2 mg/l heptadecane were achieved using glucose, Yeast malt and oatmeal media, respectively. Besides producing alkanes, we found elevated levels of internal free fatty acids and triglycerides in the alkane producing transformant. These findings can indicate that a yet unidentified, native fatty aldehyde dehydrogenase channels back the fatty aldehydes into the fatty acid metabolism, thus competing for substrate with the heterologously expressed fatty aldehyde deformylating oxygenase. These findings will potentially facilitate the future application of robust, fungal cell factories for the production of advanced biofuels from various substrates.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 32 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Netherlands 1 3%
Denmark 1 3%
Unknown 30 94%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 6 19%
Researcher 6 19%
Student > Bachelor 5 16%
Professor > Associate Professor 3 9%
Student > Master 3 9%
Other 2 6%
Unknown 7 22%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 11 34%
Agricultural and Biological Sciences 8 25%
Medicine and Dentistry 2 6%
Arts and Humanities 1 3%
Immunology and Microbiology 1 3%
Other 2 6%
Unknown 7 22%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 January 2017.
All research outputs
#4,798,932
of 8,862,686 outputs
Outputs from AMB Express
#248
of 560 outputs
Outputs of similar age
#167,465
of 301,385 outputs
Outputs of similar age from AMB Express
#23
of 52 outputs
Altmetric has tracked 8,862,686 research outputs across all sources so far. This one is in the 27th percentile – i.e., 27% of other outputs scored the same or lower than it.
So far Altmetric has tracked 560 research outputs from this source. They receive a mean Attention Score of 2.2. This one is in the 7th percentile – i.e., 7% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 301,385 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 34th percentile – i.e., 34% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 52 others from the same source and published within six weeks on either side of this one. This one is in the 5th percentile – i.e., 5% of its contemporaries scored the same or lower than it.