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Saxifragifolin D attenuates phagosome maturation arrest in Mycobacterium tuberculosis-infected macrophages via an AMPK and VPS34-dependent pathway

Overview of attention for article published in AMB Express, January 2017
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13 Mendeley
Title
Saxifragifolin D attenuates phagosome maturation arrest in Mycobacterium tuberculosis-infected macrophages via an AMPK and VPS34-dependent pathway
Published in
AMB Express, January 2017
DOI 10.1186/s13568-016-0317-6
Pubmed ID
Authors

Jia Zhou, Jia Zhou, Rui Xu, Xian-zhi Du, Xiang-dong Zhou, Qi Li

Abstract

Saxifragifolin D (SD), a traditional Chinese medicine, is a pentacyclic triterpenoid compound first isolated from Androsace umbellata. Various plant triterpenoids have been reported to exhibit antitubercular activity. In this study, THP-1-derived macrophages were infected with an attenuated M. tuberculosis (M.tb) strain, H37Ra. Intracellular replication of M.tb was evaluated by counting the colonies after 4 weeks of incubation. The results indicated that SD treatment reduced the intracellular replication of M.tb in THP-1-derived macrophages but not in A549 cells. We performed a phagosome maturation test using confocal microscopy and found that SD treatment partially attenuated the phagosome arrest induced by M.tb infection. These effects were dependent on a VPS34-associated pathway. Immunoprecipitation assays showed that SD increased intracellular UVRAG-linked VPS34, the active VPS34 complex II. However, SD had no effect on the total VPS34 pool. Moreover, the results indicated that the SD-mediated increase in VPS34 complex II activity was mediated by an AMPK-dependent pathway. Collectively, these data indicate that SD may be a promising candidate for treatment of M.tb.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Netherlands 1 8%
Unknown 12 92%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 3 23%
Student > Master 2 15%
Student > Ph. D. Student 2 15%
Unspecified 1 8%
Student > Doctoral Student 1 8%
Other 1 8%
Unknown 3 23%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 3 23%
Immunology and Microbiology 2 15%
Business, Management and Accounting 1 8%
Unspecified 1 8%
Nursing and Health Professions 1 8%
Other 3 23%
Unknown 2 15%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 January 2017.
All research outputs
#4,794,286
of 8,854,945 outputs
Outputs from AMB Express
#248
of 559 outputs
Outputs of similar age
#167,698
of 301,902 outputs
Outputs of similar age from AMB Express
#23
of 52 outputs
Altmetric has tracked 8,854,945 research outputs across all sources so far. This one is in the 27th percentile – i.e., 27% of other outputs scored the same or lower than it.
So far Altmetric has tracked 559 research outputs from this source. They receive a mean Attention Score of 2.2. This one is in the 7th percentile – i.e., 7% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 301,902 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 34th percentile – i.e., 34% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 52 others from the same source and published within six weeks on either side of this one. This one is in the 5th percentile – i.e., 5% of its contemporaries scored the same or lower than it.