| Title |
Xylanases of Cellulomonas flavigena: expression, biochemical characterization, and biotechnological potential
|
|---|---|
| Published in |
AMB Express, January 2017
|
| DOI | 10.1186/s13568-016-0308-7 |
| Pubmed ID | |
| Authors |
Alexander V. Lisov, Oksana V. Belova, Zoya A. Lisova, Nataliy G. Vinokurova, Alexey S. Nagel, Zhanna I. Andreeva-Kovalevskaya, Zhanna I. Budarina, Maxim O. Nagornykh, Marina V. Zakharova, Andrey M. Shadrin, Alexander S. Solonin, Alexey A. Leontievsky |
| Abstract |
Four xylanases of Cellulomonas flavigena were cloned, expressed in Escherichia coli and purified. Three enzymes (CFXyl1, CFXyl2, and CFXyl4) were from the GH10 family, while CFXyl3 was from the GH11 family. The enzymes possessed moderate temperature stability and a neutral pH optimum. The enzymes were more stable at alkaline pH values. CFXyl1 and CFXyl2 hydrolyzed xylan to form xylobiose, xylotriose, xylohexaose, xylopentaose, and xylose, which is typical for GH10. CFXyl3 (GH11) and CFXyl4 (GH10) formed the same xylooligosaccharides, but xylose was formed in small amounts. The xylanases made efficient saccharification of rye, wheat and oat, common components of animal feed, which indicates their high biotechnological potential. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 27 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 27 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 6 | 22% |
| Student > Master | 4 | 15% |
| Student > Bachelor | 3 | 11% |
| Researcher | 2 | 7% |
| Librarian | 1 | 4% |
| Other | 3 | 11% |
| Unknown | 8 | 30% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 7 | 26% |
| Agricultural and Biological Sciences | 5 | 19% |
| Environmental Science | 1 | 4% |
| Chemical Engineering | 1 | 4% |
| Immunology and Microbiology | 1 | 4% |
| Other | 3 | 11% |
| Unknown | 9 | 33% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 January 2017.
All research outputs
#15,416,191
of 22,925,760 outputs
Outputs from AMB Express
#445
of 1,236 outputs
Outputs of similar age
#256,817
of 421,214 outputs
Outputs of similar age from AMB Express
#20
of 46 outputs
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