| Title |
Adipose-derived human stem/stromal cells: comparative organ specific mitochondrial bioenergy profiles
|
|---|---|
| Published in |
SpringerPlus, December 2016
|
| DOI | 10.1186/s40064-016-3712-1 |
| Pubmed ID | |
| Authors |
Alice S. Ferng, Katherine M. Marsh, Jamie M. Fleming, Renee F. Conway, David Schipper, Naing Bajaj, Alana M. Connell, Tia Pilikian, Kitsie Johnson, Ray Runyan, Stephen M. Black, John A. Szivek, Zain Khalpey |
| Abstract |
Adipose-derived stem/stromal cells (ASCs) isolated from the stromal vascular fraction are a source of mesenchymal stem cells that have been shown to be beneficial in many regenerative medicine applications. ASCs are an attractive source of stem cells in particular, due to their lack of immunogenicity. This study examines differences between mitochondrial bioenergetic profiles of ASCs isolated from adipose tissue of five peri-organ regions: pericardial, thymic, knee, shoulder, and abdomen. Flow cytometry showed that the majority of each ASC population isolated from the adipose tissue of 12 donors, with an n = 3 for each tissue type, were positive for MSC markers CD90, CD73, and CD105, and negative for hematopoietic markers CD34, CD11B, CD19, and CD45. Bioenergetic profiles were obtained for ASCs with an n = 4 for each tissue type and graphed together for comparison. Mitochondrial stress tests provided the following measurements: basal respiration rate (measured as oxygen consumption rate [pmol O2/min], ATP production, proton leak, maximal respiration, respiratory control ratio, coupling efficiency, and non-mitochondrial respiration. Glycolytic stress tests provided the following measurements: basal glycolysis rate (measured as extracellular acidification rate [mpH/min]), glycolytic capacity, glycolytic reserve, and non-glycolytic acidification. The main goal of this manuscript was to provide baseline reference values for future experiments and to compare bioenergetic potentials of ASCs isolated from adipose tissue harvested from different anatomical locations. Through an investigation of mitochondrial respiration and glycolysis, it was demonstrated that bioenergetic profiles do not significantly differ by region due to depot-dependent and donor-dependent variability. Thus, although the physiological function, microenvironment and anatomical harvest site may directly affect the characteristics of ASCs isolated from different organ regions, the ultimate utility of ASCs remains independent of the anatomical harvest site. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 32 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 32 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 6 | 19% |
| Student > Bachelor | 6 | 19% |
| Student > Ph. D. Student | 5 | 16% |
| Student > Doctoral Student | 2 | 6% |
| Lecturer | 2 | 6% |
| Other | 6 | 19% |
| Unknown | 5 | 16% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 7 | 22% |
| Nursing and Health Professions | 4 | 13% |
| Agricultural and Biological Sciences | 4 | 13% |
| Engineering | 3 | 9% |
| Medicine and Dentistry | 3 | 9% |
| Other | 3 | 9% |
| Unknown | 8 | 25% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 December 2016.
All research outputs
#15,409,382
of 22,919,505 outputs
Outputs from SpringerPlus
#936
of 1,850 outputs
Outputs of similar age
#250,876
of 416,563 outputs
Outputs of similar age from SpringerPlus
#45
of 74 outputs
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