| Title |
High titer heterologous rhamnolipid production
|
|---|---|
| Published in |
AMB Express, December 2016
|
| DOI | 10.1186/s13568-016-0298-5 |
| Pubmed ID | |
| Authors |
Janina Beuker, Theresa Barth, Anke Steier, Andreas Wittgens, Frank Rosenau, Marius Henkel, Rudolf Hausmann |
| Abstract |
Heterologous mono-rhamnolipid production by Pseudomonas putida KT2440 pSynPro8oT_rhlAB using glucose as the single carbon source was characterized in fed-batch bioreactor cultivations. For the described experiments, a defined mineral salt medium was used, and a two phase glucose feeding profile was applied, which yielded a final rhamnolipid concentration of 14.9 g/L. Applying the feeding profile, glucose stayed almost constant until 28 h of cultivation and decreased afterwards to limiting levels. Until the end of cultivation 253.0 ± 0.1 g glucose was added to the bioreactor of which a total of 252.0 ± 0.6 g glucose was metabolized. By modeling the fed-batch bioreactor cultivations the time courses of generated biomass, rhamnolipid and consumed glucose were described. The model was furthermore used to derive key process parameters from the collected data. The obtained values for the specific product formation rates (qRL) reached 18 mg/(g h) and yield coefficients (YRL/S) 10 mg/g respectively. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 61 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Poland | 1 | 2% |
| Germany | 1 | 2% |
| Unknown | 59 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 14 | 23% |
| Student > Master | 9 | 15% |
| Student > Ph. D. Student | 8 | 13% |
| Researcher | 6 | 10% |
| Student > Doctoral Student | 5 | 8% |
| Other | 5 | 8% |
| Unknown | 14 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 18 | 30% |
| Agricultural and Biological Sciences | 15 | 25% |
| Engineering | 3 | 5% |
| Chemistry | 3 | 5% |
| Immunology and Microbiology | 2 | 3% |
| Other | 4 | 7% |
| Unknown | 16 | 26% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 December 2016.
All research outputs
#14,878,745
of 22,912,409 outputs
Outputs from AMB Express
#347
of 1,236 outputs
Outputs of similar age
#240,539
of 418,942 outputs
Outputs of similar age from AMB Express
#18
of 45 outputs
Altmetric has tracked 22,912,409 research outputs across all sources so far. This one is in the 33rd percentile – i.e., 33% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,236 research outputs from this source. They receive a mean Attention Score of 2.8. This one has gotten more attention than average, scoring higher than 64% of its peers.
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We're also able to compare this research output to 45 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 55% of its contemporaries.