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Functional expression and evaluation of heterologous phosphoketolases in Saccharomyces cerevisiae

Overview of attention for article published in AMB Express, November 2016
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  • Above-average Attention Score compared to outputs of the same age (64th percentile)
  • Good Attention Score compared to outputs of the same age and source (76th percentile)

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Title
Functional expression and evaluation of heterologous phosphoketolases in Saccharomyces cerevisiae
Published in
AMB Express, November 2016
DOI 10.1186/s13568-016-0290-0
Pubmed ID
Authors

Alexandra Bergman, Verena Siewers, Jens Nielsen, Yun Chen

Abstract

Phosphoketolases catalyze an energy- and redox-independent cleavage of certain sugar phosphates. Hereby, the two-carbon (C2) compound acetyl-phosphate is formed, which enzymatically can be converted into acetyl-CoA-a key precursor in central carbon metabolism. Saccharomyces cerevisiae does not demonstrate efficient phosphoketolase activity naturally. In this study, we aimed to compare and identify efficient heterologous phosphoketolase enzyme candidates that in yeast have the potential to reduce carbon loss compared to the native acetyl-CoA producing pathway by redirecting carbon flux directly from C5 and C6 sugars towards C2-synthesis. Nine phosphoketolase candidates were expressed in S. cerevisiae of which seven produced significant amounts of acetyl-phosphate after provision of sugar phosphate substrates in vitro. The candidates showed differing substrate specificities, and some demonstrated activity levels significantly exceeding those of candidates previously expressed in yeast. The conducted studies also revealed that S. cerevisiae contains endogenous enzymes capable of breaking down acetyl-phosphate, likely into acetate, and that removal of the phosphatases Gpp1 and Gpp2 could largely prevent this breakdown. An evaluation of in vivo function of a subset of phosphoketolases was conducted by monitoring acetate levels during growth, confirming that candidates showing high activity in vitro indeed showed increased acetate accumulation, but expression also decreased cellular fitness. The study shows that expression of several bacterial phosphoketolase candidates in S. cerevisiae can efficiently divert intracellular carbon flux toward C2-synthesis, thus showing potential to be used in metabolic engineering strategies aimed to increase yields of acetyl-CoA derived compounds.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 91 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 91 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 15 16%
Researcher 13 14%
Student > Ph. D. Student 13 14%
Student > Bachelor 11 12%
Student > Doctoral Student 7 8%
Other 10 11%
Unknown 22 24%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 36 40%
Agricultural and Biological Sciences 19 21%
Chemical Engineering 5 5%
Engineering 3 3%
Computer Science 1 1%
Other 3 3%
Unknown 24 26%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 November 2021.
All research outputs
#7,246,403
of 22,901,818 outputs
Outputs from AMB Express
#156
of 1,236 outputs
Outputs of similar age
#107,586
of 306,451 outputs
Outputs of similar age from AMB Express
#8
of 46 outputs
Altmetric has tracked 22,901,818 research outputs across all sources so far. This one has received more attention than most of these and is in the 67th percentile.
So far Altmetric has tracked 1,236 research outputs from this source. They receive a mean Attention Score of 2.8. This one has done well, scoring higher than 85% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 306,451 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 64% of its contemporaries.
We're also able to compare this research output to 46 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 76% of its contemporaries.