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Identification of mutations, gene expression changes and fusion transcripts by whole transcriptome RNAseq in docetaxel resistant prostate cancer cells

Overview of attention for article published in SpringerPlus, October 2016
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Title
Identification of mutations, gene expression changes and fusion transcripts by whole transcriptome RNAseq in docetaxel resistant prostate cancer cells
Published in
SpringerPlus, October 2016
DOI 10.1186/s40064-016-3543-0
Pubmed ID
Authors

Ma, Yuanjun, Miao, Yali, Peng, Zhuochun, Sandgren, Johanna, De Ståhl, Teresita Díaz, Huss, Mikael, Lennartsson, Lena, Liu, Yanling, Nistér, Monica, Nilsson, Sten, Li, Chunde, Yuanjun Ma, Yali Miao, Zhuochun Peng, Johanna Sandgren, Teresita Díaz De Ståhl, Mikael Huss, Lena Lennartsson, Yanling Liu, Monica Nistér, Sten Nilsson, Chunde Li

Abstract

Docetaxel has been the standard first-line therapy in metastatic castration resistant prostate cancer. The survival benefit is, however, limited by either primary or acquired resistance. In this study, Du145 prostate cancer cells were converted to docetaxel-resistant cells Du145-R and Du145-RB by in vitro culturing. Next generation RNAseq was employed to analyze these cell lines. Forty-two genes were identified to have acquired mutations after the resistance development, of which thirty-four were found to have mutations in published sequencing studies using prostate cancer samples from patients. Fourteen novel and 2 previously known fusion genes were inferred from the RNA-seq data, and 13 of these were validated by RT-PCR and/or re-sequencing. Four in-frame fusion transcripts could be transcribed into fusion proteins in stably transfected HEK293 cells, including MYH9-EIF3D and LDLR-RPL31P11, which were specific identified or up-regulated in the docetaxel resistant DU145 cells. A panel of 615 gene transcripts was identified to have significantly changed expression profile in the docetaxel resistant cells. These transcriptional changes have potential for further study as predictive biomarkers and as targets of docetaxel treatment.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 32 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 32 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 6 19%
Student > Ph. D. Student 6 19%
Student > Bachelor 5 16%
Researcher 4 13%
Student > Doctoral Student 2 6%
Other 3 9%
Unknown 6 19%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 7 22%
Medicine and Dentistry 7 22%
Agricultural and Biological Sciences 6 19%
Arts and Humanities 1 3%
Business, Management and Accounting 1 3%
Other 2 6%
Unknown 8 25%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 November 2016.
All research outputs
#20,351,881
of 22,899,952 outputs
Outputs from SpringerPlus
#1,460
of 1,850 outputs
Outputs of similar age
#271,300
of 313,856 outputs
Outputs of similar age from SpringerPlus
#111
of 131 outputs
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