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Microplate cell-retaining methodology for high-content analysis of individual non-adherent unanchored cells in a population

Overview of attention for article published in Biomedical Microdevices, June 2006
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  • Good Attention Score compared to outputs of the same age (70th percentile)
  • High Attention Score compared to outputs of the same age and source (80th percentile)

Mentioned by

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10 patents
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1 video uploader

Citations

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16 Dimensions

Readers on

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63 Mendeley
Title
Microplate cell-retaining methodology for high-content analysis of individual non-adherent unanchored cells in a population
Published in
Biomedical Microdevices, June 2006
DOI 10.1007/s10544-006-9143-y
Pubmed ID
Authors

Assaf Deutsch, Naomi Zurgil, Ihar Hurevich, Yana Shafran, Elena Afrimzon, Pnina Lebovich, Mordechai Deutsch

Abstract

A high throughput Microtiter plate Cell Retainer (MCR) has been developed to enable, for the first time, high-content, time-dependent analysis of the same single non-adherent and non-anchored cells in a large cell population, while bio-manipulating the cells. The identity of each cell in the investigated population is secured, even during bio-manipulation, by cell retention in a specially designed concave microlens, acting as a picoliter well (PW). The MCR technique combines micro-optical features and microtiter plate methodology. The array of PWs serves as the bottom of a microtiter plate, fitted with a unique flow damper element. The latter enables rapid fluid exchange without dislodging the cells from their original PWs, thus maintaining the cells' identity. Loading cell suspensions and reagents into the MCR is performed by simple pouring, followed by gravitational sedimentation and settling of cells into the PWs. Cell viability and cell division within the MCR were shown to be similar to those obtained under similar conditions in a standard microtiter plate. The efficiency of single cell occupancy in the MCR exceeded 90%. No cell dislodging was observed when comparing images before and after bio-manipulations (rinsing, staining, etc.). The MCR permits the performance of kinetic measurements on an individual cell basis. Data acquisition is governed by software, controlling microscope performance, stage position and image acquisition and analysis. The PW's unique micro-optical features enable rapid, simultaneous signal analysis of each individual cell, bypassing lengthy image analysis.

Mendeley readers

The data shown below were compiled from readership statistics for 63 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Israel 1 2%
United States 1 2%
Slovenia 1 2%
Unknown 60 95%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 12 19%
Researcher 10 16%
Student > Master 5 8%
Professor 1 2%
Student > Bachelor 1 2%
Other 1 2%
Unknown 33 52%
Readers by discipline Count As %
Agricultural and Biological Sciences 11 17%
Engineering 5 8%
Biochemistry, Genetics and Molecular Biology 4 6%
Chemistry 2 3%
Chemical Engineering 2 3%
Other 5 8%
Unknown 34 54%

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 May 2018.
All research outputs
#3,689,403
of 12,974,522 outputs
Outputs from Biomedical Microdevices
#115
of 486 outputs
Outputs of similar age
#41,396
of 146,509 outputs
Outputs of similar age from Biomedical Microdevices
#1
of 5 outputs
Altmetric has tracked 12,974,522 research outputs across all sources so far. This one has received more attention than most of these and is in the 71st percentile.
So far Altmetric has tracked 486 research outputs from this source. They receive a mean Attention Score of 3.8. This one has done well, scoring higher than 75% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 146,509 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 70% of its contemporaries.
We're also able to compare this research output to 5 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them