Title |
Towards an optimal and unbiased approach for tumor cell isolation
|
---|---|
Published in |
Biomedical Microdevices, March 2013
|
DOI | 10.1007/s10544-013-9757-9 |
Pubmed ID | |
Authors |
Bhuvanendran Nair Gourikutty Sajay, Chia-Pin Chang, Hamizah Ahmad, Wong Chee Chung, Poenar Daniel Puiu, Abdur Rub Abdur Rahman |
Abstract |
Our current understanding of clinical significance or the lack thereof of circulating tumor cells (CTCs) is biased by the technology used to isolate these rare cells. Despite the presence of a vast number of academic and commercial technologies, the lack of a standardized and optimized platform has been widely noted. We present a negative enrichment approach, integrating WBC depletion and chemical-free RBC depletion in the same setup without the need for centrifugation, washing or multiple sample handling steps. This approach achieves an average of >90 % recovery of spiked tumor cells and >99 % total WBC depletion in whole blood across multiple cell lines, in a simple and easy-to-use assay. The results presented herein and ongoing improvements aim to fulfill the need for a highly reliable, unbiased, standardized, and optimized CTC isolation platform, using component technologies that are validated for cell isolation. |
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Singapore | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Scientists | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Italy | 1 | 5% |
Unknown | 20 | 95% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 6 | 29% |
Student > Ph. D. Student | 5 | 24% |
Student > Doctoral Student | 2 | 10% |
Student > Master | 2 | 10% |
Professor | 1 | 5% |
Other | 2 | 10% |
Unknown | 3 | 14% |
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Engineering | 6 | 29% |
Agricultural and Biological Sciences | 4 | 19% |
Biochemistry, Genetics and Molecular Biology | 3 | 14% |
Materials Science | 3 | 14% |
Physics and Astronomy | 1 | 5% |
Other | 2 | 10% |
Unknown | 2 | 10% |