| Title |
Microwell Regulation of Pluripotent Stem Cell Self-Renewal and Differentiation
|
|---|---|
| Published in |
BioNanoScience, September 2012
|
| DOI | 10.1007/s12668-012-0050-9 |
| Pubmed ID | |
| Authors |
Cheston Hsiao, Sean P. Palecek |
| Abstract |
The fates of pluripotent stem cells (PSCs), including survival, self-renewal, and differentiation, are regulated by chemical and mechanical cues presented in the three-dimensional (3D) microenvironment. Most PSC studies have been performed on two-dimensional substrates. However, 3D culture systems have demonstrated the importance of intercellular interactions in regulating PSC self-renewal and differentiation. Microwell culture systems have been developed to generate homogenous PSC colonies of defined sizes and shapes and to study how colony morphology affects cell fate. Using microwells, researchers have demonstrated that PSCs remain in a self-renewing undifferentiated state as a result of autocrine and paracrine signaling. Other studies have shown that microwell regulation of embryoid body size affects lineage commitment during differentiation via cell-cell contact and expression of soluble signals. In this review, we discuss recent advances in the design and utilization of 3D microwell platforms for studying intercellular regulation of PSC cell fate decisions and the underlying molecular mechanisms. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 34 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Italy | 1 | 3% |
| United Kingdom | 1 | 3% |
| Singapore | 1 | 3% |
| Greece | 1 | 3% |
| United States | 1 | 3% |
| Unknown | 29 | 85% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 12 | 35% |
| Researcher | 8 | 24% |
| Student > Master | 4 | 12% |
| Student > Doctoral Student | 2 | 6% |
| Student > Bachelor | 2 | 6% |
| Other | 4 | 12% |
| Unknown | 2 | 6% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 13 | 38% |
| Engineering | 8 | 24% |
| Biochemistry, Genetics and Molecular Biology | 4 | 12% |
| Chemical Engineering | 2 | 6% |
| Mathematics | 1 | 3% |
| Other | 4 | 12% |
| Unknown | 2 | 6% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 March 2013.
All research outputs
#17,682,134
of 22,701,287 outputs
Outputs from BioNanoScience
#73
of 118 outputs
Outputs of similar age
#123,615
of 168,557 outputs
Outputs of similar age from BioNanoScience
#2
of 3 outputs
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