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Effect of textile dyes on activity and differential regulation of laccase genes from Pleurotus ostreatus grown in submerged fermentation

Overview of attention for article published in AMB Express, October 2016
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Title
Effect of textile dyes on activity and differential regulation of laccase genes from Pleurotus ostreatus grown in submerged fermentation
Published in
AMB Express, October 2016
DOI 10.1186/s13568-016-0263-3
Pubmed ID
Authors

Verónica Garrido-Bazán, Maura Téllez-Téllez, Alfredo Herrera-Estrella, Gerardo Díaz-Godínez, Soley Nava-Galicia, Miguel Ángel Villalobos-López, Analilia Arroyo-Becerra, Martha Bibbins-Martínez

Abstract

This research was conducted to extend the knowledge on the differential regulation of laccase genes in response to dyes. In order to accomplish this, we analyzed both, the expression of five laccase genes by real time RT-qPCR, and also the laccase activity and isoforms patterns during the time-course of a Pleurotus ostreatus submerged fermentation supplemented with either acetyl yellow G (AYG) or remazol brilliant blue R (RBBR) dyes. For the purpose of obtaining a stable reference gene for optimal normalization of RT-quantitative PCR gene expression assays, we tested four candidate reference genes. As a result of this analysis, gpd was selected as reference index for data normalization. The addition of dyes had an induction effect on the enzymatic activity and also modified the zymogram profile. Fermentation with RBBR showed the highest laccase activity and number of isoforms along the course of the fermentation. Laccase gene expression profiles displayed up/down regulation along the fermentation time in four laccase genes (pox4, pox3, poxa1b and pox2), while pox1 was not expressed in either of the fermentation conditions. AYG addition caused the highest induction and repression levels for genes pox3 and poxa1b respectively. The expression level for all genes in the presence of RBBR were lower than in AYG, being in both conditions this response growth time dependent. These results show the influence of the nature of dyes on the induction level of laccase activity and on the differential regulation of the laccase genes expression in P. ostreatus.

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Geographical breakdown

Country Count As %
Japan 1 2%
Unknown 40 98%

Demographic breakdown

Readers by professional status Count As %
Researcher 8 20%
Professor 6 15%
Student > Ph. D. Student 6 15%
Student > Master 4 10%
Student > Bachelor 2 5%
Other 4 10%
Unknown 11 27%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 10 24%
Agricultural and Biological Sciences 5 12%
Environmental Science 4 10%
Engineering 2 5%
Unspecified 1 2%
Other 4 10%
Unknown 15 37%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 October 2016.
All research outputs
#15,385,802
of 22,890,496 outputs
Outputs from AMB Express
#446
of 1,236 outputs
Outputs of similar age
#201,979
of 320,333 outputs
Outputs of similar age from AMB Express
#28
of 62 outputs
Altmetric has tracked 22,890,496 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,236 research outputs from this source. They receive a mean Attention Score of 2.8. This one is in the 40th percentile – i.e., 40% of its peers scored the same or lower than it.
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We're also able to compare this research output to 62 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.