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Expression, purification and immobilization of tannase from Staphylococcus lugdunensis MTCC 3614

Overview of attention for article published in AMB Express, October 2016
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Title
Expression, purification and immobilization of tannase from Staphylococcus lugdunensis MTCC 3614
Published in
AMB Express, October 2016
DOI 10.1186/s13568-016-0261-5
Pubmed ID
Authors

Amballa Chaitanyakumar, M. Anbalagan

Abstract

Enzymes find their applications in various industries, due to their error free conversion of substrate into product. Tannase is an enzyme used by various industries for degradation of tannin. Biochemical characterization of a specific enzyme from one organism to other is one of the ways to search for enzymes with better traits for industrial applications. Here, tannase encoding gene from Staphylococcus lugdunensis was cloned and suitability of the enzyme in various conditions was analysed to find its application in various industry. The recombinant protein was expressed with 6× His tag and purified using nickel affinity beads. The enzyme was purified up to homogeneity, with approximate molecular weight of 66 kDa. Purified tannase exhibited specific activity of about 716 U/mg. Optimum enzyme activity was found to be 40 °C at pH 7.0. Biochemical characterization revealed; metal ions such as Zn(2+), Fe(2+), Fe(3+) and Mn(2+) inhibited tannase activity, and SDS at lower concentration, increased tannase activity. Non polar organic solvents increased the tannase activity and polar solvents inhibited the tannase activity. Tannase immobilization studies show protection of the enzyme under wide range of pH and temperature. Also in this study we report a method for recovery and repeated use of the tannase.

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Geographical breakdown

Country Count As %
Unknown 40 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 6 15%
Student > Ph. D. Student 4 10%
Student > Postgraduate 3 8%
Student > Doctoral Student 3 8%
Student > Bachelor 3 8%
Other 8 20%
Unknown 13 33%
Readers by discipline Count As %
Agricultural and Biological Sciences 14 35%
Biochemistry, Genetics and Molecular Biology 3 8%
Chemistry 3 8%
Chemical Engineering 2 5%
Immunology and Microbiology 2 5%
Other 2 5%
Unknown 14 35%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 October 2016.
All research outputs
#15,385,802
of 22,890,496 outputs
Outputs from AMB Express
#446
of 1,236 outputs
Outputs of similar age
#201,635
of 319,862 outputs
Outputs of similar age from AMB Express
#28
of 62 outputs
Altmetric has tracked 22,890,496 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,236 research outputs from this source. They receive a mean Attention Score of 2.8. This one is in the 40th percentile – i.e., 40% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 319,862 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 28th percentile – i.e., 28% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 62 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.