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High level expression and facile purification of recombinant silk-elastin-like polymers in auto induction shake flask cultures

Overview of attention for article published in AMB Express, February 2013
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  • Above-average Attention Score compared to outputs of the same age and source (63rd percentile)

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99 Mendeley
Title
High level expression and facile purification of recombinant silk-elastin-like polymers in auto induction shake flask cultures
Published in
AMB Express, February 2013
DOI 10.1186/2191-0855-3-11
Pubmed ID
Authors

Raul Machado, João Azevedo-Silva, Cristina Correia, Tony Collins, Francisco Javier Arias, Jose Carlos Rodríguez-Cabello, Margarida Casal

Abstract

Silk-elastin-like polymers (SELPs) are protein-based polymers composed of repetitive amino acid sequence motifs found in silk fibroin (GAGAGS) and mammalian elastin (VPGVG). These polymers are of much interest, both from a fundamental and applied point of view, finding potential application in biomedicine, nanotechnology and as materials. The successful employment of such polymers in such diverse fields, however, requires the ready availability of a variety of different forms with novel enhanced properties and which can be simply prepared in large quantities on an industrial scale. In an attempt to create new polymer designs with improved properties and applicability, we have developed four novel SELPs wherein the elastomer forming sequence poly(VPGVG) is replaced with a plastic-like forming sequence, poly(VPAVG), and combined in varying proportions with the silk motif. Furthermore, we optimised a simplified production procedure for these, making use of an autoinduction medium to reduce process intervention and with the production level obtained being 6-fold higher than previously reported for other SELPs, with volumetric productivities above 150 mg/L. Finally, we took advantage of the known enhanced stability of these polymers in developing an abridged, non-chromatographic downstream processing and purification protocol. A simple acid treatment allowed for cell disruption and the obtention of relative pure SELP in one-step, with ammonium sulphate precipitation being subsequently used to enable improved purity. These simplified production and purification procedures improve process efficiency and reduce costs in the preparation of these novel polymers and enhances their potential for application.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 99 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Portugal 3 3%
India 1 1%
Unknown 95 96%

Demographic breakdown

Readers by professional status Count As %
Student > Master 23 23%
Researcher 20 20%
Student > Ph. D. Student 16 16%
Student > Bachelor 6 6%
Professor 6 6%
Other 13 13%
Unknown 15 15%
Readers by discipline Count As %
Agricultural and Biological Sciences 31 31%
Biochemistry, Genetics and Molecular Biology 20 20%
Chemistry 9 9%
Materials Science 7 7%
Engineering 6 6%
Other 5 5%
Unknown 21 21%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 31 May 2017.
All research outputs
#18,327,422
of 22,694,633 outputs
Outputs from AMB Express
#795
of 1,228 outputs
Outputs of similar age
#219,339
of 282,906 outputs
Outputs of similar age from AMB Express
#7
of 19 outputs
Altmetric has tracked 22,694,633 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,228 research outputs from this source. They receive a mean Attention Score of 2.8. This one is in the 21st percentile – i.e., 21% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 282,906 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 11th percentile – i.e., 11% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 19 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 63% of its contemporaries.