| Title |
Adaptive response of Rhodococcus opacus PWD4 to salt and phenolic stress on the level of mycolic acids
|
|---|---|
| Published in |
AMB Express, September 2016
|
| DOI | 10.1186/s13568-016-0241-9 |
| Pubmed ID | |
| Authors |
Carla C. C. R. de Carvalho, Martin A. Fischer, Sandra Kirsten, Birgit Würz, Lukas Y. Wick, Hermann J. Heipieper |
| Abstract |
Mycolata form a group of Gram-positive bacteria with unique cell envelope structures that are known for their high tolerance against antibiotics and both aromatic and aliphatic hydrocarbons. An important part of the unique surface structure of the mycolata is the presence of long chain α-alkyl-β-hydroxy fatty acids, the mycolic acids. In order to investigate the adaptive changes in the mycolic acid composition, we investigated the composition of mycolic acids during the response both to osmotic stress caused by NaCl and to 4-chlorophenol in Rhodococcus opacus PWD4. This bacterium was chosen as it is known to adapt to different kinds of stresses. In addition, it is a potential biocatalyst in bioremediation as well as for biotechnological applications. In the present study, cells of R. opacus PWD4, grown in liquid cultures, responded to toxic concentrations of NaCl by increasing the ratio between mycolic acids and membrane phospholipid fatty acids (MA/PLFA-ratio). Cells reacted to both NaCl and 4-chlorophenol by decreasing both the average chain length and the unsaturation index of their mycolic acids. These changes in mycolic acid composition correlated with increases in cell surface hydrophobicity and saturation of membrane fatty acids, demonstrating the relation between mycolic acid and phospholipid synthesis and their contribution to cell surface properties of R. opacus PWD4. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 27 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 27 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 7 | 26% |
| Researcher | 6 | 22% |
| Student > Ph. D. Student | 6 | 22% |
| Student > Doctoral Student | 1 | 4% |
| Professor > Associate Professor | 1 | 4% |
| Other | 1 | 4% |
| Unknown | 5 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 8 | 30% |
| Agricultural and Biological Sciences | 6 | 22% |
| Immunology and Microbiology | 4 | 15% |
| Engineering | 2 | 7% |
| Chemistry | 1 | 4% |
| Other | 1 | 4% |
| Unknown | 5 | 19% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 September 2016.
All research outputs
#15,383,207
of 22,886,568 outputs
Outputs from AMB Express
#446
of 1,236 outputs
Outputs of similar age
#211,711
of 332,538 outputs
Outputs of similar age from AMB Express
#25
of 53 outputs
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