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PepT1 mRNA expression levels in sea bream (Sparus aurata) fed different plant protein sources

Overview of attention for article published in SpringerPlus, January 2013
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Title
PepT1 mRNA expression levels in sea bream (Sparus aurata) fed different plant protein sources
Published in
SpringerPlus, January 2013
DOI 10.1186/2193-1801-2-17
Pubmed ID
Authors

Genciana Terova, Lidia Robaina, Marisol Izquierdo, AnnaGiulia Cattaneo, Silvia Molinari, Giovanni Bernardini, Marco Saroglia

Abstract

The expression and regulation of intestinal oligopeptide transporter (PepT)-1 when vegetable sources are used as a substitute for fish meal in the diet of marine fish has not yet been explored. In the present study, as part of our ongoing work on elucidating PepT1 gene expression in relation to different dietary treatments, we have now isolated and deposited in Genbank database (accession no. GU733710) a cDNA sequence representing the PepT1 in the sea bream (Sparus aurata). The "de novo" prediction of the three-dimensional structure of PepT1 protein is presented.We also analyzed diet-induced changes in the expression of PepT1 mRNA via real-time RT-PCR using the standard curve method. Sea bream were fed for 140 days with one of the following four diet formulations (43% protein/21% lipid): a control fast growth-promoting diet (C), and three diets with the same formulation but in which 15% of the fish meal was substituted by protein concentrates either from lupine (LPC), chick pea (CPC), or green pea (PPC). Fish fed PPC had significantly (p < 0.05) lower levels of PepT1 transcripts in the proximal intestine than the controls, whereas PepT1 transcript levels in fish fed LPC or CPC were not significantly different from the controls. Although growth was similar between fish fed with different diets during the first 72 days of feeding, growth of the fish fed with PPC was reduced during the second part of the trial and was significantly (p < 0.05) lower than fish fed LPC and CPC diets by the end of the experiment. Correlation between these results and fish growth performances highlights that the intestinal PepT1 mRNA level may serve as a useful marker of the dietary protein quality and absorption efficiency.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 54 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 1 2%
Spain 1 2%
Hungary 1 2%
Unknown 51 94%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 12 22%
Student > Master 8 15%
Student > Bachelor 6 11%
Researcher 6 11%
Student > Doctoral Student 4 7%
Other 10 19%
Unknown 8 15%
Readers by discipline Count As %
Agricultural and Biological Sciences 28 52%
Biochemistry, Genetics and Molecular Biology 7 13%
Veterinary Science and Veterinary Medicine 2 4%
Chemical Engineering 1 2%
Environmental Science 1 2%
Other 7 13%
Unknown 8 15%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 January 2013.
All research outputs
#20,178,948
of 22,693,205 outputs
Outputs from SpringerPlus
#1,461
of 1,852 outputs
Outputs of similar age
#252,401
of 285,214 outputs
Outputs of similar age from SpringerPlus
#44
of 86 outputs
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