@EpiDemos82 Depends on how good your depletion is. We have had good results with ~1-2m CDS reads per genome, but I'm mainly interested in more-abundant transcripts for my applications… Fig 2 in this paper is helpful: https://t.co/5dBxwdrSC9
@JCoutoPhoenix I’d say you always want at least 3. Another way to approach it would be to consider how much depth you need and if you can add more samples overall anyway, without reducing biological replicates per strain. This paper is a bit old but might
This is a very nice paper and a MUST for anyone considering an RNA-seq expt http://t.co/zD6aHnWchb
@BioMickWatson depth saturates for some things, e.g. RNAseq (http://t.co/nZALvAE3NP), transcriptomes (http://t.co/jPaEByPyCN)
How deep is deep enough for RNA-Seq profiling of bacterial transcriptomes? http://t.co/Uh9EBeRy @BMC_series #rnaseq
#ngs sequencing #depth analysis for #rna-seq and #de. One lane enough for monoculture bacteria. http://t.co/U2Qeoc9A (RT @genetics_blog)
How deep is deep enough for RNA-Seq profiling of bacterial transcriptomes? http://t.co/zKuHtQp5
@BioMickWatson this link point to a different article...This is the right one: http://t.co/557B6NRp
@BioMickWatson this link point to a different article...This is the right one: http://t.co/557B6NRp
RT @rnomics: Top @RNomics #tweeted story: How deep is deep enough for RNA-Seq profiling o... [BM… http://t.co/8GEwBIZb, see more http://t.co/iXmj5KPD
Top @RNomics #tweeted story: How deep is deep enough for RNA-Seq profiling o... [BM… http://t.co/8GEwBIZb, see more http://t.co/iXmj5KPD
How deep is deep enough for #RNA-Seq profiling o... [BMC Genomics. 2012] - PubMed - NCBI:... http://t.co/tMdgYeU7 #rnomics_bioinfo @MyEN
How deep is deep enough for RNA-Seq profiling of bacterial transcriptomes? http://t.co/V9fJlZOx
RT @genetics_blog: How deep is deep enough for RNA-Seq profiling of bacterial transcriptomes? http://t.co/QqJJFiv8 @myen
How deep is deep enough for RNA-Seq profiling of bacterial transcriptomes? http://t.co/QqJJFiv8 @myen
How deep is deep enough for RNA-Seq profiling of bacterial transcriptomes? http://t.co/QqJJFiv8 @myen
How deep is deep enough for RNA-Seq profiling of bacterial transcriptomes? http://t.co/DIktCZIf via @BMC_Series
How deep is deep enough for RNA-Seq profiling of bacterial transcriptomes? http://t.co/DIktCZIf via @BMC_Series
How deep is deep enough for RNA-Seq profiling of bacterial transcriptomes? http://t.co/DIktCZIf via @BMC_Series
How deep is deep enough for #RNA-Seq profiling of bacterial transcriptomes?: Background: High-throughput... http://t.co/IArRa8D5 #BMC @MyEN
How deep is deep enough for #RNASeq profiling of bacterial transcriptomes? 306Million HiSeq reads is likely overkill! @ http://t.co/hJUs1uJp
How deep is deep enough for #RNA-Seq profiling of bacterial transcriptomes?: Background: High-throughput... http://t.co/IArRa8D5 #BMC @MyEN