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Optimization of lipid production by the oleaginous yeast Lipomyces starkeyi by random mutagenesis coupled to cerulenin screening

Overview of attention for article published in AMB Express, December 2012
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Title
Optimization of lipid production by the oleaginous yeast Lipomyces starkeyi by random mutagenesis coupled to cerulenin screening
Published in
AMB Express, December 2012
DOI 10.1186/2191-0855-2-64
Pubmed ID
Authors

Eulalia Tapia V, Andréia Anschau, Alessandro LV Coradini, Telma T Franco, Ana Carolina Deckmann

Abstract

In this work we performed assays for the genetic improvement of the oleaginous yeast Lipomyces starkeyi DSM 70296 focusing on its utilization for lipid biosynthesis from renewable sources. The genetic optimization was carried out by random mutagenesis by ultraviolet irradiation and mutant selection by cerulenin, a compound displaying inhibitory effects on lipid biosynthesis. Mutants demonstrating normal growth in presence of cerulenin were considered as good candidates for further studies. Using this strategy, we selected 6 mutants for further studies, in which their productivities were evaluated by fermentation in shaken flasks and bioreactor. The evaluation of the fermentative performance of mutants was carried out using xylose as sole carbon source; the fermentation of wild-type strain was used as reference. Using this strategy it was possible to identify one mutant (termed A1) presenting a significant increase in the productivity rates of both biomass and lipid in comparison to wild-type strain. A1 mutant was further studied in bioreactor using the same fermentation parameters optimized for L. starkeyi lipid production from a mixed carbon source (xylose:glucose), as previously determined by other studies in our laboratory. A1 presented a productivity increase of 15.1% in biomass and 30.7% in lipid productivity when compared to the wild-type strain with a similar fatty acid composition, despite a slight increase (approx. 7%) on the unsaturated fraction. Our work demonstrates the feasibility of the random mutagenesis strategy coupled with mutant selection based on cerulenin screening for the genetic improvement of the oleaginous yeast L. starkeyi.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 119 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
France 2 2%
Spain 1 <1%
Brazil 1 <1%
Unknown 115 97%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 27 23%
Student > Master 16 13%
Student > Doctoral Student 12 10%
Student > Bachelor 11 9%
Researcher 11 9%
Other 21 18%
Unknown 21 18%
Readers by discipline Count As %
Agricultural and Biological Sciences 40 34%
Biochemistry, Genetics and Molecular Biology 20 17%
Environmental Science 7 6%
Chemical Engineering 6 5%
Chemistry 6 5%
Other 13 11%
Unknown 27 23%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 December 2012.
All research outputs
#20,174,175
of 22,687,320 outputs
Outputs from AMB Express
#964
of 1,228 outputs
Outputs of similar age
#246,306
of 277,751 outputs
Outputs of similar age from AMB Express
#9
of 17 outputs
Altmetric has tracked 22,687,320 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,228 research outputs from this source. They receive a mean Attention Score of 2.8. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 17 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.