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Identification of antibacterial peptides from endophytic microbiome

Overview of attention for article published in Applied Microbiology & Biotechnology, August 2016
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About this Attention Score

  • Above-average Attention Score compared to outputs of the same age (58th percentile)
  • High Attention Score compared to outputs of the same age and source (82nd percentile)

Mentioned by

twitter
5 tweeters

Citations

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7 Dimensions

Readers on

mendeley
27 Mendeley
Title
Identification of antibacterial peptides from endophytic microbiome
Published in
Applied Microbiology & Biotechnology, August 2016
DOI 10.1007/s00253-016-7765-4
Pubmed ID
Authors

M. V. Tejesvi, P. Picart, M. Kajula, H. Hautajärvi, L. Ruddock, H.H. Kristensen, A. Tossi, H.G. Sahl, S. Ek, S. Mattila, A. M. Pirttilä

Abstract

Endophytes, microorganisms living inside plant tissues, are promising producers of lead compounds for the pharmaceutical industry. However, the majority of endophytes are unculturable and therefore inaccessible for functional studies. To evaluate genetic resources of endophytes, we analyzed the biodiversity of fungal microbiome of black crowberry (Empetrum nigrum L.) by next-generation sequencing and found that it consists mainly of unknown taxa. We then separated the host and the endophyte genomes and constructed a fosmid expression library from the endophytic DNA. This library was screened for antibacterial activity against Staphylococcus aureus. A unique antibacterial clone was selected for further analysis, and a gene En-AP1 was identified with no similarity to known sequences. The expressed, folded protein En-AP1 was not active against S. aureus, while tryptic digests exhibited antimicrobial activity. Seven out of twelve synthesized peptides, predicted antibacterial in silico, exhibited in vitro activity towards both S. aureus and Escherichia coli. We propose that the En-AP1 protein is degraded in the library host E. coli and antimicrobial fragments are released from the cell, explaining the in vitro antibacterial activity of the clone. This is the first report of a novel gene expressed in vitro derived from an endophytic microbiome, demonstrating the potential of finding novel genes and compounds from unculturable endophytes.

Twitter Demographics

The data shown below were collected from the profiles of 5 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 27 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 27 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 8 30%
Student > Ph. D. Student 5 19%
Unspecified 4 15%
Student > Master 4 15%
Student > Bachelor 2 7%
Other 4 15%
Readers by discipline Count As %
Agricultural and Biological Sciences 8 30%
Biochemistry, Genetics and Molecular Biology 5 19%
Unspecified 4 15%
Chemistry 4 15%
Immunology and Microbiology 3 11%
Other 3 11%

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 August 2016.
All research outputs
#6,469,488
of 12,225,271 outputs
Outputs from Applied Microbiology & Biotechnology
#3,437
of 5,233 outputs
Outputs of similar age
#105,302
of 263,536 outputs
Outputs of similar age from Applied Microbiology & Biotechnology
#31
of 155 outputs
Altmetric has tracked 12,225,271 research outputs across all sources so far. This one is in the 46th percentile – i.e., 46% of other outputs scored the same or lower than it.
So far Altmetric has tracked 5,233 research outputs from this source. They receive a mean Attention Score of 3.6. This one is in the 34th percentile – i.e., 34% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 263,536 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 58% of its contemporaries.
We're also able to compare this research output to 155 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 82% of its contemporaries.