↓ Skip to main content

Multiplex PCR based genotypic characterization of pathogenic vancomycin resistant Enterococcus faecalis recovered from an Indian river along a city landscape

Overview of attention for article published in SpringerPlus, July 2016
Altmetric Badge

Mentioned by

twitter
1 X user

Citations

dimensions_citation
13 Dimensions

Readers on

mendeley
41 Mendeley
Title
Multiplex PCR based genotypic characterization of pathogenic vancomycin resistant Enterococcus faecalis recovered from an Indian river along a city landscape
Published in
SpringerPlus, July 2016
DOI 10.1186/s40064-016-2870-5
Pubmed ID
Authors

Pushpa Lata, Siya Ram, Rishi Shanker

Abstract

Enterococci are normal commensals of human gut, but vancomycin-resistant enterococci (VRE) are a severe threat to human health. Antimicrobial-resistant enterococci have been reported previously from Indian surface waters. However, the presence of antimicrobial resistance and virulence markers in Enterococcus faecalis, the most dominant enterococci is yet to be investigated. The goal of this study was to analyse concentration of enterococci and distribution of antimicrobial resistance and virulence markers in E. faecalis isolates from river waters along an important north Indian city landscape. We enumerated enterococci in river water samples (n = 60) collected from five sites across the Lucknow city landscape using the most probable number and membrane-filtration methods. The antimicrobial sensitivity profile of E. faecalis isolate was generated with the Kirby-Bauer antimicrobial disc diffusion assay. The multiplex PCR was used for genotypic characterization of vancomycin-resistance and virulence in E. faecalis isolates. Enterococci density (p < 0.0001) increased from up-to-down-stream sites. Multiplex PCR based genotypic characterization has shown a significant distribution of virulence-markers gelE, ace or efaA in the E. faecalis isolates (p < 0.05). The range of antimicrobial-resistance varied from 5 to 12 in the landscape with the frequency of vancomycin-resistant E. faecalis (VRE) ranging from 22 to 100 %. The occurrence of pathogenic VRE in river Gomti surface water is an important health concern. The observed high background pool of resistance and virulence in E. faecalis in river waters has the potential to disseminate more alarming antimicrobial resistance in the environment and poses serious health risk in developing countries like India as VRE infections could lead to increased cost of healthcare.

X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 41 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 41 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 7 17%
Student > Master 5 12%
Student > Ph. D. Student 5 12%
Student > Bachelor 4 10%
Student > Doctoral Student 2 5%
Other 4 10%
Unknown 14 34%
Readers by discipline Count As %
Medicine and Dentistry 5 12%
Immunology and Microbiology 5 12%
Biochemistry, Genetics and Molecular Biology 4 10%
Agricultural and Biological Sciences 3 7%
Engineering 3 7%
Other 7 17%
Unknown 14 34%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 August 2016.
All research outputs
#20,337,210
of 22,882,389 outputs
Outputs from SpringerPlus
#1,461
of 1,851 outputs
Outputs of similar age
#320,229
of 365,665 outputs
Outputs of similar age from SpringerPlus
#207
of 248 outputs
Altmetric has tracked 22,882,389 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,851 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.7. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 365,665 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 248 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.